Literature DB >> 34458439

Protein Expression Protocol for the Catalytic Domain of an Adenylate Cyclase Membrane-Anchored by a Vibrio Quorum Sensing Receptor.

Stephanie Beltz1, Joachim E Schultz1.   

Abstract

The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported ( Beltz et al., 2016 ). This protocol describes the expression and membrane preparation for these chimeric proteins.
Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Adenylate cyclase (AC); CqsS; French press; Membrane protein; Protein expression; Quorum sensing (QS)

Year:  2017        PMID： 34458439      PMCID： PMC8376520          DOI： 10.21769/BioProtoc.2112

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  Adenylyl cyclase Rv1625c of Mycobacterium tuberculosis: a progenitor of mammalian adenylyl cyclases.

Authors:  Y L Guo; T Seebacher; U Kurz; J U Linder; J E Schultz
Journal:  EMBO J       Date:  2001-07-16       Impact factor: 11.598

2.  Interaction of Rv1625c, a mycobacterial class IIIa adenylyl cyclase, with a mammalian congener.

Authors:  Ying Lan Guo; Ursula Kurz; Anita Schultz; Jürgen U Linder; Dorothea Dittrich; Christine Keller; Stefan Ehlers; Peter Sander; Joachim E Schultz
Journal:  Mol Microbiol       Date:  2005-08       Impact factor: 3.501

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Regulation by the quorum sensor from Vibrio indicates a receptor function for the membrane anchors of adenylate cyclases.

Authors:  Stephanie Beltz; Jens Bassler; Joachim E Schultz
Journal:  Elife       Date:  2016-02-27       Impact factor: 8.140
  4 in total

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