Literature DB >> 34458435

Pseudomonas syringae Flood-inoculation Method in Arabidopsis.

Yasuhiro Ishiga1, Takako Ishiga1, Yuki Ichinose2, Kirankumar S Mysore3.   

Abstract

Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis thaliana; Pathogenicity; Plant innate immunity; Pseudomonas syringae

Year:  2017        PMID： 34458435      PMCID： PMC8376610          DOI： 10.21769/BioProtoc.2106

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  NTRC and chloroplast-generated reactive oxygen species regulate Pseudomonas syringae pv. tomato disease development in tomato and Arabidopsis.

Authors:  Yasuhiro Ishiga; Takako Ishiga; Tamding Wangdi; Kirankumar S Mysore; Srinivasa Rao Uppalapati
Journal:  Mol Plant Microbe Interact       Date:  2012-03       Impact factor: 4.171

2.  Pseudomonas syringae pv. tomato OxyR Is Required for Virulence in Tomato and Arabidopsis.

Authors:  Yasuhiro Ishiga; Yuki Ichinose
Journal:  Mol Plant Microbe Interact       Date:  2016-01-11       Impact factor: 4.171

3.  Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions.

Authors:  Yasuhiro Ishiga; Takako Ishiga; Srinivasa R Uppalapati; Kirankumar S Mysore
Journal:  Plant Methods       Date:  2011-10-06       Impact factor: 4.993

4.  NADPH-dependent thioredoxin reductase C plays a role in nonhost disease resistance against Pseudomonas syringae pathogens by regulating chloroplast-generated reactive oxygen species.

Authors:  Yasuhiro Ishiga; Takako Ishiga; Yoko Ikeda; Takakazu Matsuura; Kirankumar S Mysore
Journal:  PeerJ       Date:  2016-04-26       Impact factor: 2.984
  4 in total
  2 in total

1.  Functional role of formate dehydrogenase 1 (FDH1) for host and nonhost disease resistance against bacterial pathogens.

Authors:  Seonghee Lee; Ramu S Vemanna; Sunhee Oh; Clemencia M Rojas; Youngjae Oh; Amita Kaundal; Taegun Kwon; Hee-Kyung Lee; Muthappa Senthil-Kumar; Kirankumar S Mysore
Journal:  PLoS One       Date:  2022-05-20       Impact factor: 3.752

2.  Flood inoculation of seedlings on culture medium to study interactions between Pseudomonas syringae pv. actinidiae and kiwifruit.

Authors:  Takako Ishiga; Nanami Sakata; Viet Tru Nguyen; Yasuhiro Ishiga
Journal:  J Gen Plant Pathol       Date:  2020-03-27       Impact factor: 1.449
  2 in total

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