Literature DB >> 34458431

In vitro Dephosphorylation Assay of c-Myc.

Peng Liao1, Weichao Wang1, Xin Ge2.   

Abstract

This protocol describes experimental procedures for in vitro dephosphorylation assay of human protein c-Myc. This protocol can be adapted to detect phosphatase activity of other Ser/Thr phosphatases.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Dephosphorylation; SCP1; c-Myc

Year:  2017        PMID： 34458431      PMCID： PMC8376479          DOI： 10.21769/BioProtoc.2011

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  Rapid coomassie blue staining of protein gels.

Authors:  Richard J Simpson
Journal:  Cold Spring Harb Protoc       Date:  2010-04

2.  SCP1 regulates c-Myc stability and functions through dephosphorylating c-Myc Ser62.

Authors:  W Wang; P Liao; M Shen; T Chen; Y Chen; Y Li; X Lin; X Ge; P Wang
Journal:  Oncogene       Date:  2015-04-20       Impact factor: 9.867

3.  Small C-terminal domain phosphatases dephosphorylate the regulatory linker regions of Smad2 and Smad3 to enhance transforming growth factor-beta signaling.

Authors:  Katharine H Wrighton; Danielle Willis; Jianyin Long; Fang Liu; Xia Lin; Xin-Hua Feng
Journal:  J Biol Chem       Date:  2006-10-10       Impact factor: 5.157

4.  SCP phosphatases suppress renal cell carcinoma by stabilizing PML and inhibiting mTOR/HIF signaling.

Authors:  Yu-Ching Lin; Li-Ting Lu; Hsin-Yi Chen; Xueyan Duan; Xia Lin; Xin-Hua Feng; Ming-Jer Tang; Ruey-Hwa Chen
Journal:  Cancer Res       Date:  2014-10-07       Impact factor: 12.701

5.  A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5.

Authors:  Michele Yeo; Patrick S Lin; Michael E Dahmus; Gordon N Gill
Journal:  J Biol Chem       Date:  2003-04-28       Impact factor: 5.157

6.  Small C-terminal domain phosphatase enhances snail activity through dephosphorylation.

Authors:  Yadi Wu; B Mark Evers; Binhua P Zhou
Journal:  J Biol Chem       Date:  2008-11-12       Impact factor: 5.157
  6 in total

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