Literature DB >> 34458423

Measurements of Free-swimming Speed of Motile Salmonella Cells in Liquid Media.

Yusuke V Morimoto1,2, Keiichi Namba1,2, Tohru Minamino2.   

Abstract

Bacteria such as Escherichia coli and Salmonella enterica swim in liquid media using the bacterial flagella. The flagellum consists of the basal body (rotary motor), the hook (universal joint) and the filament (helical screw). Since mutants with a defect in flagellar assembly and function cannot swim smoothly, motility assay is an easy way to characterize flagellar mutants. Here, we describe how to measure free-swimming speeds of Salmonella motile cells in liquid media. Free-swimming behavior under a microscope shows a significant variation among bacterial cells.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Bacterial flagella; Motility; Motor; Optical microscopy; Proton motive force; Salmonella

Year:  2017        PMID： 34458423      PMCID： PMC8376631          DOI： 10.21769/BioProtoc.2093

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  7 in total

1.  The speed of the flagellar rotary motor of Escherichia coli varies linearly with protonmotive force.

Authors:  Christopher V Gabel; Howard C Berg
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-11       Impact factor: 11.205

2.  Distinct roles of the FliI ATPase and proton motive force in bacterial flagellar protein export.

Authors:  Tohru Minamino; Keiichi Namba
Journal:  Nature       Date:  2008-01-24       Impact factor: 49.962

Review 3.  The bacterial flagellar motor and its structural diversity.

Authors:  Tohru Minamino; Katsumi Imada
Journal:  Trends Microbiol       Date:  2015-01-20       Impact factor: 17.079

4.  Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella.

Authors:  S Yamaguchi; H Fujita; K Sugata; T Taira; T Iino
Journal:  J Gen Microbiol       Date:  1984-02

5.  Effect of intracellular pH on rotational speed of bacterial flagellar motors.

Authors:  Tohru Minamino; Yasuo Imae; Fumio Oosawa; Yuji Kobayashi; Kenji Oosawa
Journal:  J Bacteriol       Date:  2003-02       Impact factor: 3.490

Review 6.  Structure and function of the bi-directional bacterial flagellar motor.

Authors:  Yusuke V Morimoto; Tohru Minamino
Journal:  Biomolecules       Date:  2014-02-18

7.  The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export.

Authors:  Tohru Minamino; Yusuke V Morimoto; Noritaka Hara; Phillip D Aldridge; Keiichi Namba
Journal:  PLoS Pathog       Date:  2016-03-04       Impact factor: 6.823
  7 in total
  1 in total

1.  Novel Amiloride Derivatives That Inhibit Bacterial Motility across Multiple Strains and Stator Types.

Authors:  M I Islam; J H Bae; T Ishida; P Ridone; J Lin; M J Kelso; Y Sowa; B J Buckley; M A B Baker
Journal:  J Bacteriol       Date:  2021-09-13       Impact factor: 3.490
  1 in total

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