Literature DB >> 3444989

Three-step procedure for isolation of epithelial cells from the lungs of sheep with jaagsiekte.

F A Jassim1, J M Sharp, P D Marinello.   

Abstract

An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.

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Year:  1987        PMID： 3444989

Source DB:  PubMed          Journal:  Res Vet Sci        ISSN： 0034-5288            Impact factor:   2.534


  7 in total

1.  Jaagsiekte sheep retrovirus infects multiple cell types in the ovine lung.

Authors:  Henny M Martineau; Chris Cousens; Stuart Imlach; Mark P Dagleish; David J Griffiths
Journal:  J Virol       Date:  2011-01-26       Impact factor: 5.103

2.  Regulation of surfactant protein and defensin mRNA expression in cultured ovine type II pneumocytes by all-trans retinoic acid and VEGF.

Authors:  B Grubor; D K Meyerholz; T Lazic; M M DeMacedo; R J Derscheid; J M Hostetter; J M Gallup; J C DeMartini; M R Ackermann
Journal:  Int J Exp Pathol       Date:  2006-10       Impact factor: 1.925

3.  Three-dimensional culture of an ovine pulmonary adenocarcinoma-derived cell line results in re-expression of surfactant proteins and Jaagsiekte sheep retrovirus.

Authors:  Chassidy Johnson; Hung Fan
Journal:  Virology       Date:  2011-04-09       Impact factor: 3.616

4.  Jaagsiekte retrovirus is widely distributed both in T and B lymphocytes and in mononuclear phagocytes of sheep with naturally and experimentally acquired pulmonary adenomatosis.

Authors:  M J Holland; M Palmarini; M Garcia-Goti; L Gonzalez; I McKendrick; M de las Heras; J M Sharp
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

5.  Jaagsiekte sheep retrovirus proviral clone JSRV(JS7), derived from the JS7 lung tumor cell line, induces ovine pulmonary carcinoma and is integrated into the surfactant protein A gene.

Authors:  J C DeMartini; J V Bishop; T E Allen; F A Jassim; J M Sharp; M de las Heras; D R Voelker; J O Carlson
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

6.  A large animal model to evaluate the effects of Hsp90 inhibitors for the treatment of lung adenocarcinoma.

Authors:  Mariana Varela; Matthew Golder; Fabienne Archer; Marcelo de las Heras; Caroline Leroux; Massimo Palmarini
Journal:  Virology       Date:  2007-10-24       Impact factor: 3.616

7.  Jaagsiekte sheep retrovirus infection of lung slice cultures.

Authors:  Chris Cousens; Charline Alleaume; Esther Bijsmans; Henny M Martineau; Jeanie Finlayson; Mark P Dagleish; David J Griffiths
Journal:  Retrovirology       Date:  2015-04-09       Impact factor: 4.602
  7 in total

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