Determination of chloramphenicol in swine muscle tissue using a monoclonal antibody-mediated clean-up procedure.

A rapid and specific clean-up procedure based on immunoaffinity chromatography for the high-performance liquid chromatographic determination of chloramphenicol (CAP) in swine muscle tissue at the 10 micrograms kg-1 level is described. For preparation of the columns, monoclonal antibodies against CAP were coupled to cyanogen bromide-activated Sepharose. After immunosorption of CAP from the aqueous meat extract, CAP was eluted with methanol. Mean recoveries from spiked swine muscle tissue were 70 +/- 6% (10-50 micrograms kg-1 spiking levels). The loss of CAP can be completely attributed to the extraction; no loss was observed during the antibody-mediated clean-up.