Amal Abdallah A Abo-Elmagd1,2, Dina Sabry3,4, Ebtehal Mohammed5. 1. Assistant professor of Fixed Prosthodontics, College of Dentistry Qassim University, P.O. Box 6700, Saudi Arabia. 2. Assistant professor of Fixed Prosthodontics, Faculty of Dental Surgery, Misr for Science & Technology University, Giza, Egypt. 3. Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University in Cairo, Cairo, Egypt. 4. Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. 5. Lecturer of Oral Medicine, Oral diagnosis and Periodontology, Faculty of Dentistry, Beni-Suef University, 62511 Beni Suef, Egypt.
Abstract
OBJECTIVE: To compare the effect of temporary fixed restorations to final fixed restorations on gingival health of abutment tooth by evaluating the level of IL-1β in gingival crevicular fluid (GCF) as an inflammatory biomarker. SUBJECTS AND METHODS: It was a nonrandomized prospective observational study. Samples of GCF were collected from 24 participants with provisional and permanent definitive full-coverage restorations with subgingival margin placements at three intervals for each participant: Interval 1, after preparation and immediately before cementation of temporary fixed restoration. Interval 2, after two weeks of temporization and before receiving the final fixed restoration. Interval 3, after two weeks of final fixed restorations. GCF were collected from gingival crevice of the abutment tooth by pre-prepared filter paper with dimensions of 2mmx13mm. They were prepared for IL-1β concentration assay by enzyme-linked immuno sorbent assay - analysis (ELIZA). RESULTS: Were analyzed by SAS® software, Descriptive means and medians were used and the repeated ANVOA test was applied (1st interval) showed the highest level of IL-β (13.587 ± 5.735). In 2nd interval the level of IL-β was significantly reduced (9.602 ± 3.279). While, in 3rd interval the level of IL-β was the lowest (6.293 ± 3.279). CONCLUSION: The material and technique of fabrication of both temporary and final fixed restorations are critical for gingival health. PMMA CAD-CAM based temporary restorations showed to be compatible to gingival health by decreasing the level of IL-β level, but the zirconia as a final fixed restoration showed to have optimum compatible effect on gingival health.
OBJECTIVE: To compare the effect of temporary fixed restorations to final fixed restorations on gingival health of abutment tooth by evaluating the level of IL-1β in gingival crevicular fluid (GCF) as an inflammatory biomarker. SUBJECTS AND METHODS: It was a nonrandomized prospective observational study. Samples of GCF were collected from 24 participants with provisional and permanent definitive full-coverage restorations with subgingival margin placements at three intervals for each participant: Interval 1, after preparation and immediately before cementation of temporary fixed restoration. Interval 2, after two weeks of temporization and before receiving the final fixed restoration. Interval 3, after two weeks of final fixed restorations. GCF were collected from gingival crevice of the abutment tooth by pre-prepared filter paper with dimensions of 2mmx13mm. They were prepared for IL-1β concentration assay by enzyme-linked immuno sorbent assay - analysis (ELIZA). RESULTS: Were analyzed by SAS® software, Descriptive means and medians were used and the repeated ANVOA test was applied (1st interval) showed the highest level of IL-β (13.587 ± 5.735). In 2nd interval the level of IL-β was significantly reduced (9.602 ± 3.279). While, in 3rd interval the level of IL-β was the lowest (6.293 ± 3.279). CONCLUSION: The material and technique of fabrication of both temporary and final fixed restorations are critical for gingival health. PMMA CAD-CAM based temporary restorations showed to be compatible to gingival health by decreasing the level of IL-β level, but the zirconia as a final fixed restoration showed to have optimum compatible effect on gingival health.
Authors: Fernando Zarone; Maria Irene Di Mauro; Pietro Ausiello; Gennaro Ruggiero; Roberto Sorrentino Journal: BMC Oral Health Date: 2019-07-04 Impact factor: 2.757