| Literature DB >> 3443308 |
F W Paradis1, R A Warren, D G Kilburn, R C Miller.
Abstract
The exoglucanase gene (cex) and the endoglucanase A gene (cenA) from Cellulomonas fimi were subcloned into the Escherichia coli/Brevibacterium lactofermentum shuttle vector pBK10. Both genes were expressed to five to ten times higher levels in B. lactofermentum than in E. coli, probably because these genes were expressed from C. fimi promoters. In B. lactofermentum virtually all of the enzyme activities were in the culture supernatant. This system will facilitate analysis of the expression of the C. fimi genes in and secretion of their products from a Gram-positive bacterium.Entities:
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Year: 1987 PMID： 3443308 DOI： 10.1016/0378-1119(87)90114-4
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688