Luana Silva Bittencourt1, Diehgo Tuloza da Silva2, Igor Hamoy3, Abthyllane Amaral de Carvalho2, Marcelo Francisco da Silva4, Marcela Videira5, José Carlos Tavares Carvalho6, Edilson Matos7. 1. Programa de Pós-graduação em Biodiversidade e Biotecnologia, PPGBioNorte, Universidade Federal do Amapá - UNIFAP, Rod. Juscelino Kubitschek, KM-02, Macapá, Amapá, CEP 68.903-419, Brazil. 2. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários - PPGBAIP, Universidade Federal do Pará - UFPA, Rua Augusto Corrêa, 01 Campus Universitário Do Guamá, Belém, Pará, CEP 66.075-110, Brazil. 3. Laboratório de Genética Aplicada, Universidade Federal Rural da Amazônia - UFRA, Avenida Presidente Tancredo Neves, 2501, Belém, Pará, CEP 66.077-901, Brazil. 4. Universidade Estadual da Região Tocantina do Maranhão - UEMASUL, R. Godofredo Viana, 1300 - Centro, Imperatriz - MA, Imperatriz, Maranhão, 65900-000, Brazil. 5. Laboratório de Morfofisiologia E Sanidade Animal (LABMORSA), Universidade do Estado do Amapá - UEAP, Avenida Presidente Av. Pres. Vargas, 650 - Central, Macapá, CEP 68900-000, Brazil. marcela.videira@ueap.edu.br. 6. Universidade Federal do Amapá - UNIFAP, Rod. Juscelino Kubitschek, KM-02, Macapá, Amapá, CEP 68.903-419, Brazil. 7. Laboratório de Pesquisas Carlos Azevedo, Universidade Federal Rural do Amazonas - UFRA, Av. Presidente Tancredo Neves, 2501, Belém, Pará, CEP 66.077-901, Brazil.
Abstract
PURPOSE: The class Myxozoa consists of microscopic spores that typically present uniform morphological simplifications, containing one or two sporoplasms surrounded by valve cells. The present study analysed the morphological and phylogenetic characteristics of what proved to be a new species of Ceratomyxa found in the gallbladder of Mesonauta festivus Heckel, 1840 captured on the Piririm River in the municipality of Macapá, in Amapá state, Brazil. METHODS: The fish were collected in gillnets, and were transported alive to Amapá State University in Macapá for the analysis of the organs. Crescent- or arch-shaped free spores were observed under light microscopy, containing two polar capsules and a suture line, which is typical of the morphology of the genus Ceratomyxa, contained within plasmodia that had snake-like movements. The gallbladder was conserved in 80% alcohol for the analysis of the 18S rDNA gene, and in Davidson solution for standardhistology. RESULTS: Ceratomyxa spores (n = 20) were observed in 11 of the 50 samplesanalysed, and thus had a prevalence of 22%, and had a mean length of 4.2 ± 0.5 µm, mean thickness of 22.75 ± 0.3 µm, and two polar capsules, 1.86 ± 0.3 µm long and 1.63 ± 0.1 µm thick. CONCLUSIONS: The phylogenetic analysis indicated that the new species forms a cluster with other freshwater Amazonian Ceratomyxa species, described previously. The new species represents the first description of a taxon of the genus Ceratomyxain fish from the Brazilian state of Amapá.
PURPOSE: The class Myxozoa consists of microscopic spores that typically present uniform morphological simplifications, containing one or two sporoplasms surrounded by valve cells. The present study analysed the morphological and phylogenetic characteristics of what proved to be a new species of Ceratomyxa found in the gallbladder of Mesonauta festivus Heckel, 1840 captured on the Piririm River in the municipality of Macapá, in Amapá state, Brazil. METHODS: The fish were collected in gillnets, and were transported alive to Amapá State University in Macapá for the analysis of the organs. Crescent- or arch-shaped free spores were observed under light microscopy, containing two polar capsules and a suture line, which is typical of the morphology of the genus Ceratomyxa, contained within plasmodia that had snake-like movements. The gallbladder was conserved in 80% alcohol for the analysis of the 18S rDNA gene, and in Davidson solution for standardhistology. RESULTS: Ceratomyxa spores (n = 20) were observed in 11 of the 50 samplesanalysed, and thus had a prevalence of 22%, and had a mean length of 4.2 ± 0.5 µm, mean thickness of 22.75 ± 0.3 µm, and two polar capsules, 1.86 ± 0.3 µm long and 1.63 ± 0.1 µm thick. CONCLUSIONS: The phylogenetic analysis indicated that the new species forms a cluster with other freshwater Amazonian Ceratomyxa species, described previously. The new species represents the first description of a taxon of the genus Ceratomyxain fish from the Brazilian state of Amapá.
Authors: J R Barta; D S Martin; P A Liberator; M Dashkevicz; J W Anderson; S D Feighner; A Elbrecht; A Perkins-Barrow; M C Jenkins; H D Danforth; M D Ruff; H Profous-Juchelka Journal: J Parasitol Date: 1997-04 Impact factor: 1.276
Authors: Christopher M Whipps; Robert D Adlard; Mal S Bryant; Robert J G Lester; Vanessa Findlay; Michael L Kent Journal: J Eukaryot Microbiol Date: 2003 May-Jun Impact factor: 3.346
Authors: Marcelo Francisco da Silva; Antonio Expedito Ferreira Barroso de Carvalho; Igor Hamoy; Edilson R Matos Journal: Parasitol Res Date: 2020-01-03 Impact factor: 2.289