| Literature DB >> 34426676 |
Jaeyeaon Cho1,2, Sangsung Kim2, Hyein Lee2, Woongchan Rah2, Hee Cheol Cho3, Nam Kyun Kim3, Seongho Bae1, Dong Hoon Shin2, Min Goo Lee4, In-Hyun Park5, Yoshiaki Tanaka6, Eric Shin1, Hong Yi7, Ji Woong Han1, Patrick Tae Joon Hwang8, Ho-Wook Jun8, Hun-Jun Park9, Kyuwon Cho1, Sang Wook Lee1, Jae Kyung Jung1, Rebecca D Levit1, Mark A Sussman10,11, Richard P Harvey12, Young-Sup Yoon13,14.
Abstract
Fibroblasts can be directly reprogrammed into cardiomyocytes, endothelial cells or smooth muscle cells. Here we report the reprogramming of mouse tail-tip fibroblasts simultaneously into cells resembling these three cell types using the microRNA mimic miR-208b-3p, ascorbic acid and bone morphogenetic protein 4, as well as the formation of tissue-like structures formed by the directly reprogrammed cells. Implantation of the formed cardiovascular tissue into the infarcted hearts of mice led to the migration of reprogrammed cells to the injured tissue, reducing regional cardiac strain and improving cardiac function. The migrated endothelial cells and smooth muscle cells contributed to vessel formation, and the migrated cardiomyocytes, which initially displayed immature characteristics, became mature over time and formed gap junctions with host cardiomyocytes. Direct reprogramming of somatic cells to make cardiac tissue may aid the development of applications in cell therapy, disease modelling and drug discovery for cardiovascular diseases.Entities:
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Year: 2021 PMID： 34426676 PMCID： PMC8809198 DOI： 10.1038/s41551-021-00783-0
Source DB: PubMed Journal: Nat Biomed Eng ISSN： 2157-846X Impact factor: 25.671