| Literature DB >> 3442328 |
B L Somani1, J Khanade, R Sinha.
Abstract
Two milliliters of a reagent consisting of anthrone and tryptophan each at a 0.01% concentration in 75% sulfuric acid, when added to 0.9 ml of solution containing D-fructose, produced on heating (55 degrees C, 90 min) a pink color (lambdamax 520 nm) with an absorbance of 0.009 A/nmol. The absorbance is about three times higher than that of the standard anthrone-sulfuric acid reagent. Glucose yields a color only about 1% as intense as that yielded by fructose. The method is useful for the estimation of fructose in the presence of proteins. Synthetic Amadori compounds and glycosylated proteins containing ketoamine-linked fructose, however, were unreactive.Entities:
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Year: 1987 PMID： 3442328 DOI： 10.1016/0003-2697(87)90172-2
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365