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Literature DB >> 3442324

Rapid isoelectric focusing in a vertical polyacrylamide minigel system.

E F Robertson¹, H K Dannelly, P J Malloy, H C Reeves.

Abstract

A rapid method is described for the resolution of proteins employing isoelectric focusing in a vertical polyacrylamide minigel system. Isoelectric focusing can be performed in only 3 h, utilizing low voltage, under either native conditions or denaturing conditions in the presence of 8 M urea. The procedure permits the application of larger sample volumes containing more protein than other isoelectric focusing procedures, and provides the additional advantages of slab gels over tube gels for analytical purposes. The procedure is also well adapted for use in two-dimensional electrophoretic techniques, making it possible to complete a two-dimensional gel in 1 day.

Entities: Chemical

Mesh：

Substances：
Proteins
Urea

Year: 1987 PMID： 3442324 DOI： 10.1016/0003-2697(87)90166-7

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

67 in total

1. Functional complementation of an Escherichia coli gap mutant supports an amphibolic role for NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechocystis sp. strain PCC 6803.

Authors: F Valverde; M Losada; A Serrano
Journal: J Bacteriol Date: 1997-07 Impact factor: 3.490

2. A Purified Zinc Protease of Pea Chloroplasts, EP1, Degrades the Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase.

Authors: T. P. Bushnell; D. Bushnell; A. T. Jagendorf
Journal: Plant Physiol Date: 1993-10 Impact factor: 8.340

3. Expression and Localization of Plant Protein Disulfide Isomerase.

Authors: B. S. Shorrosh; J. Subramaniam; K. R. Schubert; R. A. Dixon
Journal: Plant Physiol Date: 1993-11 Impact factor: 8.340

4. Cytochrome c6-like protein as a putative donor of electrons to photosystem I in the cyanobacterium Nostoc sp. PCC 7119.

Authors: Francisco M Reyes-Sosa; Jorge Gil-Martínez; Fernando P Molina-Heredia
Journal: Photosynth Res Date: 2011-10-09 Impact factor: 3.573

10. Isolation and properties of two forms of thrombin inhibitor from the nymphs of the camel tick Hyalomma dromedarii (Acari: Ixodidae).

Authors: M A Ibrahim; A H Ghazy; T Maharem; M Khalil
Journal: Exp Appl Acarol Date: 2001 Impact factor: 2.132

Rapid isoelectric focusing in a vertical polyacrylamide minigel system.

1. Functional complementation of an Escherichia coli gap mutant supports an amphibolic role for NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechocystis sp. strain PCC 6803.

2. A Purified Zinc Protease of Pea Chloroplasts, EP1, Degrades the Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase.

3. Expression and Localization of Plant Protein Disulfide Isomerase.

4. Cytochrome c6-like protein as a putative donor of electrons to photosystem I in the cyanobacterium Nostoc sp. PCC 7119.

5. Water deficit triggers phospholipase D activity in the resurrection plant Craterostigma plantagineum.

6. Catalase Is Differentially Expressed in Dividing and Nondividing Protoplasts.

7. Multiple Forms of Phospholipase D following Germination and during Leaf Development of Castor Bean.

8. Comparison of polyphenol oxidase expression in glandular trichomes of solanum and lycopersicon species.

9. Circulating immune complexes and analysis of renal immune deposits in feline immunodeficiency virus-infected cats.

10. Isolation and properties of two forms of thrombin inhibitor from the nymphs of the camel tick Hyalomma dromedarii (Acari: Ixodidae).