Literature DB >> 344199

Quantitative phagocytosis by human polymorphonuclear leucocytes. Use of radiolabelled emulsions to measure thae rate of phagocytosis.

A Forsgren, D Schmeling, O Zettervall.   

Abstract

A new micro-method for the quantitative measurement of phagocytosis by neutrophils is described. The material used for phagocytosis consists of a radioactive oil emulsion coated with E. coli lipopolysaccharide. Uptake of radioactive material is a function of cell number, duration of incubation, dilution of serum used for opsonization, content of lipopolysaccharide and concentration of emulsion. This method can be used to quantify rapidly and precisely phagocytosis rates of as few as 5 x 10(4)-10(6) polymorphonuclear leucocytes and the opsonic activity of 10 microliter serum.

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Year:  1977        PMID： 344199      PMCID： PMC1445469     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  19 in total

1.  STUDIES ON HEAT-LABILE OPSONIN IN RABBIT SERUM.

Authors:  J G HIRSCH; B STRAUSS
Journal:  J Immunol       Date:  1964-01       Impact factor: 5.422

2.  PARTICLE UPTAKE BY POLYMORPHONUCLEAR LEUCOCYTES AND EHRLICH ASCITES-CARCINOMA CELLS.

Authors:  J ROBERTS; J H QUASTEL
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

3.  FACTORS INFLUENCING THE PHAGOCYTOSIS OF ESCHERICHIA COLI.

Authors:  W BRUMFITT; A A GLYNN; A PERCIVAL
Journal:  Br J Exp Pathol       Date:  1965-04

4.  Phagocytosis by guinea pig splenic cells of Escherichia coli and protein-coated bentonite particles labeled with iodine-125.

Authors:  R R Carpenter
Journal:  J Immunol       Date:  1966-06       Impact factor: 5.422

5.  Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system.

Authors:  R R MacGregor; P J Spagnuolo; A L Lentnek
Journal:  N Engl J Med       Date:  1974-09-26       Impact factor: 91.245

6.  Studies on neutrophil function. II. Pathological aspects.

Authors:  R Penny; D A Galton
Journal:  Br J Haematol       Date:  1966-09       Impact factor: 6.998

7.  Isolation and properties of phagocytic vesicles from polymorphonuclear leukocytes.

Authors:  T P Stossel; T D Pollard; R J Mason; M Vaughan
Journal:  J Clin Invest       Date:  1971-08       Impact factor: 14.808

8.  Quantitative studies of phagocytosis by polymorphonuclear leukocytes: use of emulsions to measure the initial rate of phagocytosis.

Authors:  T P Stossel; R J Mason; J Hartwig; M Vaughan
Journal:  J Clin Invest       Date:  1972-03       Impact factor: 14.808

9.  Interactions between rabbit polymorphonuclear leucocytes and staphylococci.

Authors:  Z A COHN; S I MORSE
Journal:  J Exp Med       Date:  1959-09-01       Impact factor: 14.307

10.  The fate of bacteria within phagocytic cells. I. The degradation of isotopically labeled bacteria by polymorphonuclear leucocytes and macrophages.

Authors:  Z A COHN
Journal:  J Exp Med       Date:  1963-01-01       Impact factor: 14.307
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  1 in total

1.  Pathogenesis of foreign body infection. Evidence for a local granulocyte defect.

Authors:  W Zimmerli; P D Lew; F A Waldvogel
Journal:  J Clin Invest       Date:  1984-04       Impact factor: 14.808
  1 in total

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