| Literature DB >> 34416169 |
Pin Xu1, Stefano Berto1, Ashwinikumar Kulkarni1, Byeongha Jeong1, Chryshanthi Joseph1, Kimberly H Cox1, Michael E Greenberg2, Tae-Kyung Kim1, Genevieve Konopka1, Joseph S Takahashi3.
Abstract
The suprachiasmatic nucleus (SCN) is the master circadian pacemaker in mammals and is entrained by environmental light. However, the molecular basis of the response of the SCN to light is not fully understood. We used RNA/chromatin immunoprecipitation/single-nucleus sequencing with circadian behavioral assays to identify mouse SCN cell types and explore their responses to light. We identified three peptidergic cell types that responded to light in the SCN: arginine vasopressin (AVP), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK). In each cell type, light-responsive subgroups were enriched for expression of neuronal Per-Arnt-Sim (PAS) domain protein 4 (NPAS4) target genes. Further, mice lacking Npas4 had a longer circadian period under constant conditions, a damped phase response curve to light, and reduced light-induced gene expression in the SCN. Our data indicate that NPAS4 is necessary for normal transcriptional responses to light in the SCN and critical for photic phase-shifting of circadian behavior.Entities:
Keywords: NPAS4; light simulation; single-nucleus RNA sequencing; suprachiasmatic nucleus
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Year: 2021 PMID： 34416169 PMCID： PMC8542585 DOI： 10.1016/j.neuron.2021.07.026
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 18.688