Nan Tang1, Yu-Yao Zhang1, Jue-Hua Cheng1, Zhi-Bai Zhao1, Yuan Fan1. 1. Dept. of Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China.
Abstract
OBJECTIVES: This study aimed to determine whether a correlation existed between CXC chemokine ligand 10 (CXCL10)-CXC chemokine receptor 3 (CXCR3) and CC chemokine ligand 17 (CCL17)-CC chemokine receptor 4 (CCR4) in the pathogenesis of oral lichen planus (OLP). METHODS: Peripheral blood of OLP patients (non-erosive and erosive groups) and healthy controls were collected, and T cells were isolated and purified. T cells were co-cultured with three groups: blank, anti-CXCR3, and anti-CCR4. CXCR3 and CCR4 expression were detected by flow cytometry, and CXCL10 and CCL17 were detected by enzyme-linked immunosorbent assay, respectively. RESULTS: The purities of T cells were all >95% in the three groups (P>0.05). Receptor expression showed that CXCR3 and CCR4 in the anti-CXCR3 group was downregulated in OLP compared with the blank group (P>0.05). The level of CCR4 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCR3 was upregulated (P>0.05). Ligand analysis results showed that CXCL10 in the anti-CXCR3 group was significantly downregulated in OLP compared with the blank group (P<0.05), and CCL17 was also downregulated (P>0.05). CCL17 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCL10 was upregulated (P>0.05). The trend of receptors and ligands in controls was consistent with OLP, but no significant difference existed between the antagonistic and the blank groups (P>0.05). CONCLUSIONS: Two axes interact with each other in the pathogenesis of OLP and may play different roles in its occurrence and development.
OBJECTIVES: This study aimed to determine whether a correlation existed between CXC chemokine ligand 10 (CXCL10)-CXC chemokine receptor 3 (CXCR3) and CC chemokine ligand 17 (CCL17)-CC chemokine receptor 4 (CCR4) in the pathogenesis of oral lichen planus (OLP). METHODS: Peripheral blood of OLP patients (non-erosive and erosive groups) and healthy controls were collected, and T cells were isolated and purified. T cells were co-cultured with three groups: blank, anti-CXCR3, and anti-CCR4. CXCR3 and CCR4 expression were detected by flow cytometry, and CXCL10 and CCL17 were detected by enzyme-linked immunosorbent assay, respectively. RESULTS: The purities of T cells were all >95% in the three groups (P>0.05). Receptor expression showed that CXCR3 and CCR4 in the anti-CXCR3 group was downregulated in OLP compared with the blank group (P>0.05). The level of CCR4 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCR3 was upregulated (P>0.05). Ligand analysis results showed that CXCL10 in the anti-CXCR3 group was significantly downregulated in OLP compared with the blank group (P<0.05), and CCL17 was also downregulated (P>0.05). CCL17 in the anti-CCR4 group was significantly downregulated (P<0.05), and CXCL10 was upregulated (P>0.05). The trend of receptors and ligands in controls was consistent with OLP, but no significant difference existed between the antagonistic and the blank groups (P>0.05). CONCLUSIONS: Two axes interact with each other in the pathogenesis of OLP and may play different roles in its occurrence and development.
Entities:
Keywords:
CC chemokine ligand 17; CC chemokine receptor 4; CXC chemokine ligand 10; CXC chemokine receptor 3; oral lichen planus
Authors: J-N Hayashida; S Nakamura; T Toyoshima; M Moriyama; M Sasaki; E Kawamura; Y Ohyama; W Kumamaru; K Shirasuna Journal: Bone Marrow Transplant Date: 2012-06-04 Impact factor: 5.483
Authors: Mayra B C Maymone; Robert O Greer; Jeffery Kesecker; Priya Cherukuri Sahitya; Lauren K Burdine; Anh-Dao Cheng; Alexandre C Maymone; Neelam A Vashi Journal: J Am Acad Dermatol Date: 2018-11-14 Impact factor: 11.527
Authors: Thais B Bertolini; Annie R Piñeros; Rafael Q Prado; Ana Flávia Gembre; Leandra N Z Ramalho; José Carlos Alves-Filho; Vânia L D Bonato Journal: Cell Death Dis Date: 2018-12-21 Impact factor: 8.469