| Literature DB >> 3440019 |
T Ishimine1, M Tadano, T Fukunaga, Y Okuno.
Abstract
An improved micromethod for infectivity assays and neutralization (N) tests of dengue (DEN) type 1-4 viruses was developed, using 96-well plates and the PAP (peroxidase-antiperoxidase) staining technique. The foci formed on BHK-21 cell monolayers in wells of the plate were readily countable under an ordinary stereomicroscope. This micromethod has the advantages over the micromethod of the Lab-Tek 8 chamber slide system of lower cost, requirement for smaller volumes of test sera and applicability to larger number of serum specimens for N tests of DEN viruses.Entities:
Mesh：
Year: 1987 PMID： 3440019
Source DB: PubMed Journal: Biken J ISSN： 0006-2324