Taher Elmi1, Mehdi Shafiee Ardestani2, Manijeh Motevalian3, Ali Kalantari Hesari4, Mohammad Seyyed Hamzeh2, Zahra Zamani5, Fatemeh Tabatabaie6. 1. Department of Laboratory Science, Babol Branch, Islamic Azad University, Babol, Iran. 2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 3. Department of Pharmacology and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. 4. Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran. 5. Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran. zamani@pasteur.ac.ir. 6. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. tabatabaei.f@iums.ac.ir.
Abstract
BACKGROUND: Malaria is a parasitic lethal disease caused by Plasmodium protozoa. The resistance and drugs' side effects have led to numerous researches for alternative suitable drugs with better efficiency and lower toxicity PURPOSE: In the present study, we investigated in vivo antimalarial effects of G2 linear dendrimer-based nano-chloroquine. METHODS: After the preparation of nano dendrimer G2, chloroquine loading was done. Determine the characterization of particles were specified by DLS, SLS and SEM. The LC-MS and FTIR were used for verifying the nano dendrimer G2 and the loading of chloroquine into the compound. The Solubility N-chloroquine and measurement of drug release rate were done. Antiplasmodial activity of N-chloroquine on BALB/c mice was performed by the microscope and enzymatic methods. At the end, In vivo toxicity of N-chloroquine on tissues was assayed. The RBC morphology and enzyme levels were identified. RESULTS: The results showed the synthesized N-chloroquine had suitable size and solubility. Highest inhibitory effect on Plasmodium parasitic growth was observed at 16 mg/kg dose of N-chloroquine, which eliminated 95% of the parasites (p > 0.05). ED50 is observed at 7.7 mg/kg of N-chloroquine dose. Biochemical findings showed the synthesized N-chloroquine was safer than chloroquine. The N-chloroquine no adverse effects were observed in examined tissues. CONCLUSION: Due to the better effect of the synthesized N-chloroquine on Plasmodium berghei in mice compared to chloroquine, this nanoparticle can be considered as an effective anti-plasmodium compound while more comprehensive research is recommended.
BACKGROUND: Malaria is a parasitic lethal disease caused by Plasmodium protozoa. The resistance and drugs' side effects have led to numerous researches for alternative suitable drugs with better efficiency and lower toxicity PURPOSE: In the present study, we investigated in vivo antimalarial effects of G2 linear dendrimer-based nano-chloroquine. METHODS: After the preparation of nano dendrimer G2, chloroquine loading was done. Determine the characterization of particles were specified by DLS, SLS and SEM. The LC-MS and FTIR were used for verifying the nano dendrimer G2 and the loading of chloroquine into the compound. The Solubility N-chloroquine and measurement of drug release rate were done. Antiplasmodial activity of N-chloroquine on BALB/c mice was performed by the microscope and enzymatic methods. At the end, In vivo toxicity of N-chloroquine on tissues was assayed. The RBC morphology and enzyme levels were identified. RESULTS: The results showed the synthesized N-chloroquine had suitable size and solubility. Highest inhibitory effect on Plasmodium parasitic growth was observed at 16 mg/kg dose of N-chloroquine, which eliminated 95% of the parasites (p > 0.05). ED50 is observed at 7.7 mg/kg of N-chloroquine dose. Biochemical findings showed the synthesized N-chloroquine was safer than chloroquine. The N-chloroquine no adverse effects were observed in examined tissues. CONCLUSION: Due to the better effect of the synthesized N-chloroquine on Plasmodium berghei in mice compared to chloroquine, this nanoparticle can be considered as an effective anti-plasmodium compound while more comprehensive research is recommended.
Authors: Mohammad Shafiee Alavidjeh; Ismaeil Haririan; Mohammad Reza Khorramizadeh; Zohre Zarei Ghane; Mehdi Shafiee Ardestani; Hassan Namazi Journal: J Mater Sci Mater Med Date: 2010-01-16 Impact factor: 3.896
Authors: Zaid O Ibraheem; Roslaini Abd Majid; Hasidah Mohd Sidek; Sabariah Md Noor; Mun Fei Yam; Mohammad Faruq Abd Rachman Isnadi; Rusliza Basir Journal: Evid Based Complement Alternat Med Date: 2019-12-13 Impact factor: 2.629