Literature DB >> 34395805

Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis.

Yuping Cai1, Nan Liu1, Zheng-Jiang Zhu1.   

Abstract

Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment protocol in Drosophila melanogaster using stable-isotope-labeled 13C-glucose tracer followed by liquid chromatography-mass spectrometry (LC-MS) analysis. Detailed experimental setup, data acquisition and analysis are provided to facilitate the application of in vivo metabolic labeling analysis that might be applied in a wide range of biological studies.
Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  13C-glucose tracer ; Drosophila melanogaster; Liquid chromatography-mass spectrometry; Metabolic analysis; Qualitative analysis; Stable-isotope labeling

Year:  2018        PMID： 34395805      PMCID： PMC8328633          DOI： 10.21769/BioProtoc.3015

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  Epigenetic drift of H3K27me3 in aging links glycolysis to healthy longevity in Drosophila.

Authors:  Zaijun Ma; Hui Wang; Yuping Cai; Han Wang; Kongyan Niu; Xiaofen Wu; Huanhuan Ma; Yun Yang; Wenhua Tong; Feng Liu; Zhandong Liu; Yaoyang Zhang; Rui Liu; Zheng-Jiang Zhu; Nan Liu
Journal:  Elife       Date:  2018-05-29       Impact factor: 8.140

2.  Quantitative Analysis of NAD Synthesis-Breakdown Fluxes.

Authors:  Ling Liu; Xiaoyang Su; William J Quinn; Sheng Hui; Kristin Krukenberg; David W Frederick; Philip Redpath; Le Zhan; Karthikeyani Chellappa; Eileen White; Marie Migaud; Timothy J Mitchison; Joseph A Baur; Joshua D Rabinowitz
Journal:  Cell Metab       Date:  2018-05-01       Impact factor: 27.287

Review 3.  A roadmap for interpreting (13)C metabolite labeling patterns from cells.

Authors:  Joerg M Buescher; Maciek R Antoniewicz; Laszlo G Boros; Shawn C Burgess; Henri Brunengraber; Clary B Clish; Ralph J DeBerardinis; Olivier Feron; Christian Frezza; Bart Ghesquiere; Eyal Gottlieb; Karsten Hiller; Russell G Jones; Jurre J Kamphorst; Richard G Kibbey; Alec C Kimmelman; Jason W Locasale; Sophia Y Lunt; Oliver D K Maddocks; Craig Malloy; Christian M Metallo; Emmanuelle J Meuillet; Joshua Munger; Katharina Nöh; Joshua D Rabinowitz; Markus Ralser; Uwe Sauer; Gregory Stephanopoulos; Julie St-Pierre; Daniel A Tennant; Christoph Wittmann; Matthew G Vander Heiden; Alexei Vazquez; Karen Vousden; Jamey D Young; Nicola Zamboni; Sarah-Maria Fendt
Journal:  Curr Opin Biotechnol       Date:  2015-02-28       Impact factor: 9.740

Review 4.  Metabolomics and Isotope Tracing.

Authors:  Cholsoon Jang; Li Chen; Joshua D Rabinowitz
Journal:  Cell       Date:  2018-05-03       Impact factor: 41.582

5.  Metabolite concentrations, fluxes and free energies imply efficient enzyme usage.

Authors:  Junyoung O Park; Sara A Rubin; Yi-Fan Xu; Daniel Amador-Noguez; Jing Fan; Tomer Shlomi; Joshua D Rabinowitz
Journal:  Nat Chem Biol       Date:  2016-05-02       Impact factor: 15.040
  5 in total
  1 in total

1.  Global stable-isotope tracing metabolomics reveals system-wide metabolic alternations in aging Drosophila.

Authors:  Ruohong Wang; Yandong Yin; Jingshu Li; Hongmiao Wang; Wanting Lv; Yang Gao; Tangci Wang; Yedan Zhong; Zhiwei Zhou; Yuping Cai; Xiaoyang Su; Nan Liu; Zheng-Jiang Zhu
Journal:  Nat Commun       Date:  2022-06-20       Impact factor: 17.694
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.