Literature DB >> 34395802

Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.

Bingbing Ren1, Ahmed Mahmoud Mohammed Sayed2, Hwei Ling Tan1,3, Yu Keung Mok2, Ee Sin Chen1,3.   

Abstract

Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Bio-layer interferometry; Biosensor; Histone post-translational modification; Label free; Peptide; Protein interaction; Recombinant protein

Year:  2018        PMID： 34395802      PMCID： PMC8328593          DOI： 10.21769/BioProtoc.3012

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  20 in total

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Authors:  J A Bornhorst; J J Falke
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

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Review 4.  Signals and combinatorial functions of histone modifications.

Authors:  Tamaki Suganuma; Jerry L Workman
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

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Review 6.  Ten principles of heterochromatin formation and function.

Authors:  Robin C Allshire; Hiten D Madhani
Journal:  Nat Rev Mol Cell Biol       Date:  2017-12-13       Impact factor: 94.444

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8.  Proteomics to study DNA-bound and chromatin-associated gene regulatory complexes.

Authors:  Michael Wierer; Matthias Mann
Journal:  Hum Mol Genet       Date:  2016-07-11       Impact factor: 6.150

Review 9.  Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development.

Authors:  Rejane L Petersen
Journal:  Biosensors (Basel)       Date:  2017-10-31

10.  Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast.

Authors:  Bingbing Ren; Hwei Ling Tan; Thi Thuy Trang Nguyen; Ahmed Mahmoud Mohammed Sayed; Ying Li; Yu-Keung Mok; Henry Yang; Ee Sin Chen
Journal:  Nucleic Acids Res       Date:  2018-01-09       Impact factor: 16.971
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