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Literature DB >> 34395800

Fabrication and use of the dual-flow-RootChip for the imaging of Arabidopsis roots in asymmetric microenvironments.

Claire E Stanley¹, Jagriti Shrivastava^2,3, Rik Brugman², Elisa Heinzelmann², Viktoria Frajs², Andreas Bühler¹, Dirk van Swaay⁴, Guido Grossmann^2,5.

Abstract

This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact with their environment by simulating environmental heterogeneity. The goal of this protocol is to provide a detailed resource for researchers in the biological sciences wishing to employ the dfRootChip in particular, or microfluidic devices in general, in their laboratory.

Entities: Chemical

Keywords: Calcium signaling; Cell-cell communication; Dual-flow-RootChip; Environmental sensing; Lab-on-a-chip; Microfluidics; Plant-microbe interactions; Root development; Root hairs

Year: 2018 PMID： 34395800 PMCID： PMC8328679 DOI： 10.21769/BioProtoc.3010

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

16 in total

1. The RootChip: an integrated microfluidic chip for plant science.

Authors: Guido Grossmann; Woei-Jiun Guo; David W Ehrhardt; Wolf B Frommer; Rene V Sit; Stephen R Quake; Matthias Meier
Journal: Plant Cell Date: 2011-12-20 Impact factor: 11.277

Review 2. Soil-on-a-Chip: microfluidic platforms for environmental organismal studies.

Authors: Claire E Stanley; Guido Grossmann; Xavier Casadevall i Solvas; Andrew J deMello
Journal: Lab Chip Date: 2016-01-21 Impact factor: 6.799

3. ALFIN-LIKE 6 is involved in root hair elongation during phosphate deficiency in Arabidopsis.

Authors: Nulu Naga Prafulla Chandrika; Kalaipandian Sundaravelpandian; Su-May Yu; Wolfgang Schmidt
Journal: New Phytol Date: 2013-02-25 Impact factor: 10.151

4. Microbiome-on-a-Chip: New Frontiers in Plant-Microbiota Research.

Authors: Claire E Stanley; Marcel G A van der Heijden
Journal: Trends Microbiol Date: 2017-05-24 Impact factor: 17.079

5. Live imaging of root-bacteria interactions in a microfluidics setup.

Authors: Hassan Massalha; Elisa Korenblum; Sergey Malitsky; Orr H Shapiro; Asaph Aharoni
Journal: Proc Natl Acad Sci U S A Date: 2017-03-27 Impact factor: 11.205

Review 6. Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms.

Authors: Matthew M Crane; Kwanghun Chung; Jeffrey Stirman; Hang Lu
Journal: Lab Chip Date: 2010-04-09 Impact factor: 6.799

7. Fiji: an open-source platform for biological-image analysis.

Authors: Johannes Schindelin; Ignacio Arganda-Carreras; Erwin Frise; Verena Kaynig; Mark Longair; Tobias Pietzsch; Stephan Preibisch; Curtis Rueden; Stephan Saalfeld; Benjamin Schmid; Jean-Yves Tinevez; Daniel James White; Volker Hartenstein; Kevin Eliceiri; Pavel Tomancak; Albert Cardona
Journal: Nat Methods Date: 2012-06-28 Impact factor: 28.547

8. Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance.

Authors: Shuping Xing; Dietmar Gerald Mehlhorn; Niklas Wallmeroth; Lisa Yasmin Asseck; Ritwika Kar; Alessa Voss; Philipp Denninger; Vanessa Aphaia Fiona Schmidt; Markus Schwarzländer; York-Dieter Stierhof; Guido Grossmann; Christopher Grefen
Journal: Proc Natl Acad Sci U S A Date: 2017-01-17 Impact factor: 11.205