Literature DB >> 34395791

Analysis of the effect of Sphingomyelinase on rubellavirus infectivity in two cell lines.

Noriyuki Otsuki1, Masafumi Sakata1, Yoshio Mori1, Kiyoko Okamoto1, Makoto Takeda1.   

Abstract

Rubella is a mildly contagious disease characterized by low-grade fever and a morbilliform rash caused by the rubella virus (RuV). Viruses often use cellular phospholipids for infection. We studied the roles of cellular sphingomyelin in RuV infection. Treatment of cells with sphingomyelinase (SMase) inhibited RuV infection in rabbit kidney-derived RK13 cells and African green monkey (Cercopithecus aethiops) kidney-derived Vero cells. Our data further demonstrated that RuV used cellular sphingomyelin and cholesterol for its binding to cells and membrane fusion at the step of virus entry. Detailed protocols of our assays, which assess the effects of SMase treatment on RuV infectivity in RK13 and Vero cells, are described.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Lipid; Rubella virus; Sphingomyelin; Sphingomyelinase; Virus entry

Year:  2018        PMID： 34395791      PMCID： PMC8328626          DOI： 10.21769/BioProtoc.2992

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  Identification of the myelin oligodendrocyte glycoprotein as a cellular receptor for rubella virus.

Authors:  Haolong Cong; Yue Jiang; Po Tien
Journal:  J Virol       Date:  2011-08-31       Impact factor: 5.103

2.  Role of membrane phospholipids and glycolipids in the Vero cell surface receptor for rubella virus.

Authors:  P Mastromarino; L Cioè; S Rieti; N Orsi
Journal:  Med Microbiol Immunol       Date:  1990       Impact factor: 3.402

3.  Short self-interacting N-terminal region of rubella virus capsid protein is essential for cooperative actions of capsid and nonstructural p150 proteins.

Authors:  Masafumi Sakata; Noriyuki Otsuki; Kiyoko Okamoto; Masaki Anraku; Misato Nagai; Makoto Takeda; Yoshio Mori
Journal:  J Virol       Date:  2014-07-23       Impact factor: 5.103

4.  Elucidation of the full genetic information of Japanese rubella vaccines and the genetic changes associated with in vitro and in vivo vaccine virus phenotypes.

Authors:  Noriyuki Otsuki; Hitoshi Abo; Toru Kubota; Yoshio Mori; Yukiko Umino; Kiyoko Okamoto; Makoto Takeda; Katsuhiro Komase
Journal:  Vaccine       Date:  2011-01-18       Impact factor: 3.641

5.  Recombinant wild-type measles virus containing a single N481Y substitution in its haemagglutinin cannot use receptor CD46 as efficiently as that having the haemagglutinin of the Edmonston laboratory strain.

Authors:  Fumio Seki; Makoto Takeda; Hiroko Minagawa; Yusuke Yanagi
Journal:  J Gen Virol       Date:  2006-06       Impact factor: 3.891

6.  Binding sites for rubella virus on erythrocyte membrane.

Authors:  P Mastromarino; S Rieti; L Cioè; N Orsi
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

7.  Measles viruses on throat swabs from measles patients use signaling lymphocytic activation molecule (CDw150) but not CD46 as a cellular receptor.

Authors:  N Ono; H Tatsuo; Y Hidaka; T Aoki; H Minagawa; Y Yanagi
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

8.  Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2.

Authors:  Yuta Shirogane; Makoto Takeda; Masaharu Iwasaki; Nobuhisa Ishiguro; Hiroki Takeuchi; Yuichiro Nakatsu; Maino Tahara; Hideaki Kikuta; Yusuke Yanagi
Journal:  J Virol       Date:  2008-06-18       Impact factor: 5.103

9.  Both Sphingomyelin and Cholesterol in the Host Cell Membrane Are Essential for Rubella Virus Entry.

Authors:  Noriyuki Otsuki; Masafumi Sakata; Kyoko Saito; Kiyoko Okamoto; Yoshio Mori; Kentaro Hanada; Makoto Takeda
Journal:  J Virol       Date:  2017-12-14       Impact factor: 5.103

10.  Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro.

Authors:  Quang Duy Trinh; Ngan Thi Kim Pham; Kazuhide Takada; Shihoko Komine-Aizawa; Satoshi Hayakawa
Journal:  Viruses       Date:  2018-01-04       Impact factor: 5.048
  10 in total

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