Literature DB >> 34395766

Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus elongatus PCC 7942.

Ryudo Ohbayashi1, Hirofumi Yoshikawa2, Satoru Watanabe2.   

Abstract

Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942 also contains multiple chromosomal copies per cell at all stages of its cell cycle. Here, we describe a method for the direct visualization of multicopy chromosomes in S. elongatus 7942 by fluorescence in situ hybridization (FISH).
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Chromosome distribution; Cyanobacteria; In situ hybridization ; Polyploidy; Replication origin

Year:  2018        PMID： 34395766      PMCID： PMC8328676          DOI： 10.21769/BioProtoc.2958

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  Light-dependent and asynchronous replication of cyanobacterial multi-copy chromosomes.

Authors:  Satoru Watanabe; Ryudo Ohbayashi; Yuh Shiwa; Aska Noda; Yu Kanesaki; Taku Chibazakura; Hirofumi Yoshikawa
Journal:  Mol Microbiol       Date:  2012-02       Impact factor: 3.501

2.  Ploidy in cyanobacteria.

Authors:  Marco Griese; Christian Lange; Jörg Soppa
Journal:  FEMS Microbiol Lett       Date:  2011-09-06       Impact factor: 2.742

3.  Spatial ordering of chromosomes enhances the fidelity of chromosome partitioning in cyanobacteria.

Authors:  Isha H Jain; Vikram Vijayan; Erin K O'Shea
Journal:  Proc Natl Acad Sci U S A       Date:  2012-08-06       Impact factor: 11.205

4.  ParA-like protein influences the distribution of multi-copy chromosomes in cyanobacterium Synechococcus elongatus PCC 7942.

Authors:  Satoru Watanabe; Aska Noda; Ryudo Ohbayashi; Kana Uchioke; Ami Kurihara; Shizuka Nakatake; Sayumi Morioka; Yu Kanesaki; Taku Chibazakura; Hirofumi Yoshikawa
Journal:  Microbiology       Date:  2017-11-21       Impact factor: 2.777

5.  Control of macromolecular composition and cell division in the blue-green algae Anacystis nidulans.

Authors:  N Mann; N G Carr
Journal:  J Gen Microbiol       Date:  1974-08

6.  Cell Cycle Regulation in Marine Synechococcus sp. Strains.

Authors:  B J Binder; S W Chisholm
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

7.  Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis strain PCC 6803.

Authors:  J Labarre; F Chauvat; P Thuriaux
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

8.  Spatial and temporal organization of chromosome duplication and segregation in the cyanobacterium Synechococcus elongatus PCC 7942.

Authors:  Anna H Chen; Bruno Afonso; Pamela A Silver; David F Savage
Journal:  PLoS One       Date:  2012-10-24       Impact factor: 3.240

9.  Intensive DNA Replication and Metabolism during the Lag Phase in Cyanobacteria.

Authors:  Satoru Watanabe; Ryudo Ohbayashi; Yu Kanesaki; Natsumi Saito; Taku Chibazakura; Tomoyoshi Soga; Hirofumi Yoshikawa
Journal:  PLoS One       Date:  2015-09-02       Impact factor: 3.240
  9 in total

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