Literature DB >> 34395756

BMV Propagation, Extraction and Purification Using Chromatographic Methods.

Aleksander Strugała1, Paulina Bierwagen1, Jakub Dalibor Rybka2, Michał Giersig2,3, Marek Figlerowicz1,4,5, Anna Urbanowicz1,6.   

Abstract

Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and size-exclusion chromatography. The presented method is of similar efficiency to previously described protocols relying on differential centrifugation and can easily be scaled up. The resulting BMV capsids are stable and monodisperse and can be used for further applications.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  BMV; Brome mosaic virus; Capsid; Plant virus purification; Virion; Virus-like particles (VLP)

Year:  2018        PMID： 34395756      PMCID： PMC8328587          DOI： 10.21769/BioProtoc.2935

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  17 in total

1.  Homologous crossovers among molecules of brome mosaic bromovirus RNA1 or RNA2 segments in vivo.

Authors:  Anna Urbanowicz; Magdalena Alejska; Piotr Formanowicz; Jacek Blazewicz; Marek Figlerowicz; Jozef J Bujarski
Journal:  J Virol       Date:  2005-05       Impact factor: 5.103

2.  Characterization of a novel 5' subgenomic RNA3a derived from RNA3 of Brome mosaic bromovirus.

Authors:  Rafal Wierzchoslawski; Anna Urbanowicz; Aleksandra Dzianott; Marek Figlerowicz; Jozef J Bujarski
Journal:  J Virol       Date:  2006-09-27       Impact factor: 5.103

3.  The Molecular Weight and Other Biophysical Properties of Bromegrass Mosaic Virus.

Authors:  L E Bockstahler; P Kaesberg
Journal:  Biophys J       Date:  1962-01       Impact factor: 4.033

4.  Structure-based design and experimental engineering of a plant virus nanoparticle for the presentation of immunogenic epitopes and as a drug carrier.

Authors:  Caterina Arcangeli; Patrizia Circelli; Marcello Donini; Alaa A A Aljabali; Eugenio Benvenuto; George P Lomonossoff; Carla Marusic
Journal:  J Biomol Struct Dyn       Date:  2013-05-15

5.  Role of RNA structure in non-homologous recombination between genomic molecules of brome mosaic virus.

Authors:  M Figlerowicz
Journal:  Nucleic Acids Res       Date:  2000-04-15       Impact factor: 16.971

6.  The tripartite virions of the brome mosaic virus have distinct physical properties that affect the timing of the infection process.

Authors:  Robert Vaughan; Brady Tragesser; Peng Ni; Xiang Ma; Bogdan Dragnea; C Cheng Kao
Journal:  J Virol       Date:  2014-03-26       Impact factor: 5.103

7.  Optical Characteristics and Tumor Imaging Capabilities of Near Infrared Dyes in Free and Nano-Encapsulated Formulations Comprised of Viral Capsids.

Authors:  Yadir Guerrero; Sheela P Singh; Turong Mai; Ravoori K Murali; Leela Tanikella; Atta Zahedi; Vikas Kundra; Bahman Anvari
Journal:  ACS Appl Mater Interfaces       Date:  2017-05-31       Impact factor: 9.229

Review 8.  The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein.

Authors:  C Cheng Kao; Peng Ni; Masarapu Hema; Xinlei Huang; Bogdan Dragnea
Journal:  Mol Plant Pathol       Date:  2010-11-25       Impact factor: 5.663

9.  Optical nano-constructs composed of genome-depleted brome mosaic virus doped with a near infrared chromophore for potential biomedical applications.

Authors:  Bongsu Jung; Ayala L N Rao; Bahman Anvari
Journal:  ACS Nano       Date:  2011-01-06       Impact factor: 15.881

10.  The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.

Authors:  Peng Ni; Robert C Vaughan; Brady Tragesser; Haley Hoover; C Cheng Kao
Journal:  J Mol Biol       Date:  2013-09-13       Impact factor: 5.469
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