Literature DB >> 34395750

Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9.

Mariana Schuster1, Christine Trippel1, Petra Happel1, Daniel Lanver1, Stefanie Reißmann1, Regine Kahmann1.   

Abstract

The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis. Furthermore, we describe the necessary steps required for generating edited clonal populations, losing the Cas9 containing plasmid, and for selecting the desired clones.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  CRISPR-Cas9; Gene editing; Multiplexing; Smut fungi; Ustilago maydis

Year:  2018        PMID： 34395750      PMCID： PMC8328660          DOI： 10.21769/BioProtoc.2928

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  21 in total

1.  The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif.

Authors:  B Schulz; F Banuett; M Dahl; R Schlesinger; W Schäfer; T Martin; I Herskowitz; R Kahmann
Journal:  Cell       Date:  1990-01-26       Impact factor: 41.582

Review 2.  CRISPR system in filamentous fungi: Current achievements and future directions.

Authors:  Huaxiang Deng; Ruijie Gao; Xiangru Liao; Yujie Cai
Journal:  Gene       Date:  2017-06-16       Impact factor: 3.688

Review 3.  mRNA transport in fungal top models.

Authors:  Dierk Niessing; Ralf-Peter Jansen; Thomas Pohlmann; Michael Feldbrügge
Journal:  Wiley Interdiscip Rev RNA       Date:  2017-10-10       Impact factor: 9.957

4.  Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions.

Authors:  Kristin Bösch; Lamprinos Frantzeskakis; Miroslav Vraneš; Jörg Kämper; Kerstin Schipper; Vera Göhre
Journal:  J Vis Exp       Date:  2016-09-30       Impact factor: 1.355

5.  Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis.

Authors:  Jörg Kämper; Regine Kahmann; Michael Bölker; Li-Jun Ma; Thomas Brefort; Barry J Saville; Flora Banuett; James W Kronstad; Scott E Gold; Olaf Müller; Michael H Perlin; Han A B Wösten; Ronald de Vries; José Ruiz-Herrera; Cristina G Reynaga-Peña; Karen Snetselaar; Michael McCann; José Pérez-Martín; Michael Feldbrügge; Christoph W Basse; Gero Steinberg; Jose I Ibeas; William Holloman; Plinio Guzman; Mark Farman; Jason E Stajich; Rafael Sentandreu; Juan M González-Prieto; John C Kennell; Lazaro Molina; Jan Schirawski; Artemio Mendoza-Mendoza; Doris Greilinger; Karin Münch; Nicole Rössel; Mario Scherer; Miroslav Vranes; Oliver Ladendorf; Volker Vincon; Uta Fuchs; Björn Sandrock; Shaowu Meng; Eric C H Ho; Matt J Cahill; Kylie J Boyce; Jana Klose; Steven J Klosterman; Heine J Deelstra; Lucila Ortiz-Castellanos; Weixi Li; Patricia Sanchez-Alonso; Peter H Schreier; Isolde Häuser-Hahn; Martin Vaupel; Edda Koopmann; Gabi Friedrich; Hartmut Voss; Thomas Schlüter; Jonathan Margolis; Darren Platt; Candace Swimmer; Andreas Gnirke; Feng Chen; Valentina Vysotskaia; Gertrud Mannhaupt; Ulrich Güldener; Martin Münsterkötter; Dirk Haase; Matthias Oesterheld; Hans-Werner Mewes; Evan W Mauceli; David DeCaprio; Claire M Wade; Jonathan Butler; Sarah Young; David B Jaffe; Sarah Calvo; Chad Nusbaum; James Galagan; Bruce W Birren
Journal:  Nature       Date:  2006-11-02       Impact factor: 49.962

6.  The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis.

Authors:  Daniel Lanver; André N Müller; Petra Happel; Gabriel Schweizer; Fabian B Haas; Marek Franitza; Clément Pellegrin; Stefanie Reissmann; Janine Altmüller; Stefan A Rensing; Regine Kahmann
Journal:  Plant Cell       Date:  2018-01-25       Impact factor: 11.277

Review 7.  Early studies on recombination and DNA repair in Ustilago maydis.

Authors:  Robin Holliday
Journal:  DNA Repair (Amst)       Date:  2004-06-03

8.  DNA targeting specificity of RNA-guided Cas9 nucleases.

Authors:  Patrick D Hsu; David A Scott; Joshua A Weinstein; F Ann Ran; Silvana Konermann; Vineeta Agarwala; Yinqing Li; Eli J Fine; Xuebing Wu; Ophir Shalem; Thomas J Cradick; Luciano A Marraffini; Gang Bao; Feng Zhang
Journal:  Nat Biotechnol       Date:  2013-07-21       Impact factor: 54.908

Review 9.  The homologous recombination system of Ustilago maydis.

Authors:  William K Holloman; Jan Schirawski; Robin Holliday
Journal:  Fungal Genet Biol       Date:  2008-05-23       Impact factor: 3.495

10.  Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Authors:  Yanfang Fu; Jeffry D Sander; Deepak Reyon; Vincent M Cascio; J Keith Joung
Journal:  Nat Biotechnol       Date:  2014-01-26       Impact factor: 54.908
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