Literature DB >> 34395745

Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL).

Anna Worthmann1, Clara John1, Joerg Heeren1.   

Abstract

The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Cholesterol; Lipid metabolism; Lipid/Cholesterol biodistribution; Oral fat tolerance; Organ uptake; Radioactive tracer study

Year:  2018        PMID： 34395745      PMCID： PMC8328672          DOI： 10.21769/BioProtoc.2916

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  Real-time magnetic resonance imaging and quantification of lipoprotein metabolism in vivo using nanocrystals.

Authors:  Oliver T Bruns; Harald Ittrich; Kersten Peldschus; Michael G Kaul; Ulrich I Tromsdorf; Joachim Lauterwasser; Marija S Nikolic; Birgit Mollwitz; Martin Merkel; Nadja C Bigall; Sameer Sapra; Rudolph Reimer; Heinz Hohenberg; Horst Weller; Alexander Eychmüller; Gerhard Adam; Ulrike Beisiegel; Joerg Heeren
Journal:  Nat Nanotechnol       Date:  2009-01-25       Impact factor: 39.213

2.  Biodistribution of 4-[(14)C]cholesterol-AmBisome following a single intravenous administration to rats.

Authors:  R W Townsend; A Zutshi; I Bekersky
Journal:  Drug Metab Dispos       Date:  2001-05       Impact factor: 3.922

3.  Cold-induced conversion of cholesterol to bile acids in mice shapes the gut microbiome and promotes adaptive thermogenesis.

Authors:  Anna Worthmann; Clara John; Malte C Rühlemann; Miriam Baguhl; Femke-Anouska Heinsen; Nicola Schaltenberg; Markus Heine; Christian Schlein; Ioannis Evangelakos; Chieko Mineo; Markus Fischer; Maura Dandri; Claus Kremoser; Ludger Scheja; Andre Franke; Philip W Shaul; Joerg Heeren
Journal:  Nat Med       Date:  2017-06-12       Impact factor: 53.440

4.  Reevaluation and application of the dual-isotope plasma ratio method for the measurement of intestinal cholesterol absorption in the hamster.

Authors:  S D Turley; M W Herndon; J M Dietschy
Journal:  J Lipid Res       Date:  1994-02       Impact factor: 5.922

5.  Validation of a dual-isotope plasma ratio method for measurement of cholesterol absorption in rats.

Authors:  D B Zilversmit; L B Hughes
Journal:  J Lipid Res       Date:  1974-09       Impact factor: 5.922

6.  Brown fat activation reduces hypercholesterolaemia and protects from atherosclerosis development.

Authors:  Jimmy F P Berbée; Mariëtte R Boon; P Padmini S J Khedoe; Alexander Bartelt; Christian Schlein; Anna Worthmann; Sander Kooijman; Geerte Hoeke; Isabel M Mol; Clara John; Caroline Jung; Nadia Vazirpanah; Linda P J Brouwers; Philip L S M Gordts; Jeffrey D Esko; Pieter S Hiemstra; Louis M Havekes; Ludger Scheja; Joerg Heeren; Patrick C N Rensen
Journal:  Nat Commun       Date:  2015-03-10       Impact factor: 14.919
  6 in total

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