Literature DB >> 34395742

FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans.

Ranjan Devkota1, Marc Pilon1.   

Abstract

FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes in certain mutants.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  C. elegans; FRAP; Fatty acid; Membrane fluidity; PAQR-2; Prenylated GFP

Year:  2018        PMID： 34395742      PMCID： PMC8328621          DOI： 10.21769/BioProtoc.2913

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

Review 1.  From fixed to FRAP: measuring protein mobility and activity in living cells.

Authors:  E A Reits; J J Neefjes
Journal:  Nat Cell Biol       Date:  2001-06       Impact factor: 28.824

2.  Fatty acid composition of membrane bilayers: importance of diet polyunsaturated fat balance.

Authors:  Sarah K Abbott; Paul L Else; Taleitha A Atkins; A J Hulbert
Journal:  Biochim Biophys Acta       Date:  2012-01-18

Review 3.  Maintenance of C. elegans.

Authors:  Theresa Stiernagle
Journal:  WormBook       Date:  2006-02-11

Review 4.  Membrane lipids: where they are and how they behave.

Authors:  Gerrit van Meer; Dennis R Voelker; Gerald W Feigenson
Journal:  Nat Rev Mol Cell Biol       Date:  2008-02       Impact factor: 94.444

5.  Statins inhibit protein lipidation and induce the unfolded protein response in the non-sterol producing nematode Caenorhabditis elegans.

Authors:  Catarina Mörck; Louise Olsen; Caroline Kurth; Annelie Persson; Nadia Jin Storm; Emma Svensson; John-Olov Jansson; Marika Hellqvist; Annika Enejder; Nils J Faergeman; Marc Pilon
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-13       Impact factor: 11.205

6.  Caenorhabditis elegans PAQR-2 and IGLR-2 Protect against Glucose Toxicity by Modulating Membrane Lipid Composition.

Authors:  Emma Svensk; Ranjan Devkota; Marcus Ståhlman; Parmida Ranji; Manish Rauthan; Fredrik Magnusson; Sofia Hammarsten; Maja Johansson; Jan Borén; Marc Pilon
Journal:  PLoS Genet       Date:  2016-04-15       Impact factor: 5.917

7.  13C- and 15N-Labeling Strategies Combined with Mass Spectrometry Comprehensively Quantify Phospholipid Dynamics in C. elegans.

Authors:  Blair C R Dancy; Shaw-Wen Chen; Robin Drechsler; Philip R Gafken; Carissa Perez Olsen
Journal:  PLoS One       Date:  2015-11-03       Impact factor: 3.240

Review 8.  Revisiting the membrane-centric view of diabetes.

Authors:  Marc Pilon
Journal:  Lipids Health Dis       Date:  2016-09-27       Impact factor: 3.876

9.  The adiponectin receptor AdipoR2 and its Caenorhabditis elegans homolog PAQR-2 prevent membrane rigidification by exogenous saturated fatty acids.

Authors:  Ranjan Devkota; Emma Svensk; Mario Ruiz; Marcus Ståhlman; Jan Borén; Marc Pilon
Journal:  PLoS Genet       Date:  2017-09-08       Impact factor: 5.917
  9 in total
  2 in total

Review 1.  Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.

Authors:  Pavak Shah; Zhirong Bao; Ronen Zaidel-Bar
Journal:  Genetics       Date:  2022-07-30       Impact factor: 4.402

2.  A genetic titration of membrane composition in Caenorhabditis elegans reveals its importance for multiple cellular and physiological traits.

Authors:  Ranjan Devkota; Delaney Kaper; Rakesh Bodhicharla; Marcus Henricsson; Jan Borén; Marc Pilon
Journal:  Genetics       Date:  2021-08-26       Impact factor: 4.402
  2 in total

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