Xinyue Mao1, Chang Wang1,2, Zhihui Xu1, Yixin He1, Yanpei Hou1, Bing Li1,2. 1. Department of Nephrology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, China. 2. Department of Nephrology, 2nd Affiliated Hospital of Hainan Medical University, Haikou, China.
Abstract
INTRODUCTION: In animal models, it can be difficult to confirm therapeutic effects due to technical inconsistencies and other reasons. Although renal biopsy is widely used in clinical diagnosis, it is rarely used in animal experimental models, especially in mice, because the problems of surgery-induced renal injury and bleeding have not been solved. METHODS: We developed an easy-to-use method of renal biopsy in mice and evaluated whether 3 consecutive renal biopsies can be performed in the same kidney in a standardized manner. This method was verified using 2 mouse models, a healthy mouse model and a unilateral ureteral obstruction (UUO) mouse model, and evaluated based on renal function and histological changes. RESULTS: There were no perioperative complications in any of the model mice. There was no significant difference in serum Cr, 24-h urine protein, or kidney/body weight ratio between the biopsy and control groups. Each biopsy sample contained sufficient renal tissue. The damage to the operated tissue was limited to the tissue near the biopsy site. Compared with renal tissues in the corresponding control group, the renal tissues obtained from the 3 biopsies (healthy model days 0, 4, and 7 and UUO model days 3, 7, and 10) and the remnant renal tissues after the biopsy showed no significant difference in the glomerular sclerosis index, degree of renal tubular damage, inflammatory response and renal fibrosis in these 2 models. CONCLUSIONS: Our new standardized method of renal biopsy in mice is a safe and cost-saving approach that allows repeated renal biopsies and ensures minimal trauma and sufficient sample size to quality in experimental disease models.
INTRODUCTION: In animal models, it can be difficult to confirm therapeutic effects due to technical inconsistencies and other reasons. Although renal biopsy is widely used in clinical diagnosis, it is rarely used in animal experimental models, especially in mice, because the problems of surgery-induced renal injury and bleeding have not been solved. METHODS: We developed an easy-to-use method of renal biopsy in mice and evaluated whether 3 consecutive renal biopsies can be performed in the same kidney in a standardized manner. This method was verified using 2 mouse models, a healthy mouse model and a unilateral ureteral obstruction (UUO) mouse model, and evaluated based on renal function and histological changes. RESULTS: There were no perioperative complications in any of the model mice. There was no significant difference in serum Cr, 24-h urine protein, or kidney/body weight ratio between the biopsy and control groups. Each biopsy sample contained sufficient renal tissue. The damage to the operated tissue was limited to the tissue near the biopsy site. Compared with renal tissues in the corresponding control group, the renal tissues obtained from the 3 biopsies (healthy model days 0, 4, and 7 and UUO model days 3, 7, and 10) and the remnant renal tissues after the biopsy showed no significant difference in the glomerular sclerosis index, degree of renal tubular damage, inflammatory response and renal fibrosis in these 2 models. CONCLUSIONS: Our new standardized method of renal biopsy in mice is a safe and cost-saving approach that allows repeated renal biopsies and ensures minimal trauma and sufficient sample size to quality in experimental disease models.
Authors: Nicholas Rooney; Susan M Mason; Laura McDonald; J Henry M Däbritz; Kirsteen J Campbell; Ann Hedley; Steven Howard; Dimitris Athineos; Colin Nixon; William Clark; Joshua D G Leach; Owen J Sansom; Joanne Edwards; Ewan R Cameron; Karen Blyth Journal: Cancer Res Date: 2020-03-10 Impact factor: 12.701
Authors: Holger Schirutschke; Lars Gladrow; Christian Norkus; Simon Paul Parmentier; Bernd Hohenstein; Christian P M Hugo Journal: PLoS One Date: 2014-12-15 Impact factor: 3.240
Authors: Andinet Enquobahrie; Sam Horvath; Sreekanth Arikatla; Avi Rosenberg; Kevin Cleary; Karun Sharma Journal: Healthc Technol Lett Date: 2019-11-26