Guillaume Levenson1, Arthur Berger2, Jonathan Demma3, Guillaume Perrod4, Thomas Domet5, Lousineh Arakelian5, Patrick Bruneval6, Chloe Broudin6, Mohamed Jarraya7, Niclas Setterblad8, Gabriel Rahmi4, Jerome Larghero5, Pierre Cattan9, Lionel Faivre10, Tigran Poghosyan11. 1. Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Department de Chirurgie Viscérale, Oncologique, et Endocrinienne, Paris, France; INSERM U976 et CIC-BT501, Université de Paris, Hôpital Saint-Louis, Paris, France. Electronic address: https://twitter.com/Levenson_G. 2. Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Service de Gastroentérologie, Paris, France. Electronic address: https://twitter.com/bergerarthur7. 3. Hadassah Medical Center, Service de Chirurgie Générale, Université Hébraïque de Jerusalem, Jerusalem, Israel. 4. Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Service de Gastroentérologie, Paris, France. 5. INSERM U976 et CIC-BT501, Université de Paris, Hôpital Saint-Louis, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Unité de Thérapie Cellulaire, Paris, France. 6. Department of Pathology, Georges-Pompidou European hospital, AP-HP and Université de Paris, Paris, France. 7. Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Banque de Tissus Humains, Paris, France. 8. Plateforme technologique de l'IRSL/ Technological Core Facility, Saint-Louis Research Institute, Saint-louis Hospital, Université de Paris. 9. Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Department de Chirurgie Viscérale, Oncologique, et Endocrinienne, Paris, France; INSERM U976 et CIC-BT501, Université de Paris, Hôpital Saint-Louis, Paris, France. Electronic address: pierre.cattan@aphp.fr. 10. INSERM U976 et CIC-BT501, Université de Paris, Hôpital Saint-Louis, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Unité de Thérapie Cellulaire, Paris, France. Electronic address: https://twitter.com/FaivreLionel1. 11. INSERM U976 et CIC-BT501, Université de Paris, Hôpital Saint-Louis, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Service de Chirugie Viscérale et Oncologique, Paris, France. Electronic address: https://twitter.com/PoghosyanTigra1.
Abstract
BACKGROUND: Tissue engineering is an attractive alternative to conventional esophageal replacement techniques using intra-abdominal organs which are associated with a substantial morbidity. The objective was to evaluate the feasibility of esophageal replacement by an allogenic decellularized esophagus in a porcine model. Secondary objectives were to evaluate the benefit of decellularized esophagus recellularization with autologous bone marrow mesenchymal stromal cells and omental maturation of the decellularized esophagus. METHODS: Eighteen pigs divided into 4 experimental groups according to mesenchymal stromal cells recellularization and omental maturation underwent a 5-cm long circumferential replacement of the thoracic esophagus. Turbo green florescent protein labelling was used for in vivo mesenchymal stromal cells tracking. The graft area was covered by a stent for 3 months. Clinical and histologic outcomes were analyzed over a 6-month period. RESULTS: The median follow-up was 112 days [5; 205]. Two animals died during the first postoperative month, 2 experienced an anastomotic leakage, 13 experienced a graft area stenosis following stent migration of which 3 were sacrificed as initially planned after successful endoscopic treatment. The stent could be removed in 2 animals: the graft area showed a continuous mucosa without stenosis. After 3 months, the graft area showed a tissue specific regeneration with a mature epithelium and muscular cells. Clinical and histologic results were similar across experimental groups. CONCLUSION: Circumferential esophageal replacement by a decellularized esophagus was feasible and allowed tissue remodeling toward an esophageal phenotype. We could not demonstrate any benefit provided by the omental maturation of the decellularized esophagus nor its recellularization with mesenchymal stromal cells.
BACKGROUND: Tissue engineering is an attractive alternative to conventional esophageal replacement techniques using intra-abdominal organs which are associated with a substantial morbidity. The objective was to evaluate the feasibility of esophageal replacement by an allogenic decellularized esophagus in a porcine model. Secondary objectives were to evaluate the benefit of decellularized esophagus recellularization with autologous bone marrow mesenchymal stromal cells and omental maturation of the decellularized esophagus. METHODS: Eighteen pigs divided into 4 experimental groups according to mesenchymal stromal cells recellularization and omental maturation underwent a 5-cm long circumferential replacement of the thoracic esophagus. Turbo green florescent protein labelling was used for in vivo mesenchymal stromal cells tracking. The graft area was covered by a stent for 3 months. Clinical and histologic outcomes were analyzed over a 6-month period. RESULTS: The median follow-up was 112 days [5; 205]. Two animals died during the first postoperative month, 2 experienced an anastomotic leakage, 13 experienced a graft area stenosis following stent migration of which 3 were sacrificed as initially planned after successful endoscopic treatment. The stent could be removed in 2 animals: the graft area showed a continuous mucosa without stenosis. After 3 months, the graft area showed a tissue specific regeneration with a mature epithelium and muscular cells. Clinical and histologic results were similar across experimental groups. CONCLUSION: Circumferential esophageal replacement by a decellularized esophagus was feasible and allowed tissue remodeling toward an esophageal phenotype. We could not demonstrate any benefit provided by the omental maturation of the decellularized esophagus nor its recellularization with mesenchymal stromal cells.
Authors: Silvia Barbon; Andrea Biccari; Elena Stocco; Giovanni Capovilla; Edoardo D'Angelo; Martina Todesco; Deborah Sandrin; Andrea Bagno; Filippo Romanato; Veronica Macchi; Raffaele De Caro; Marco Agostini; Stefano Merigliano; Michele Valmasoni; Andrea Porzionato Journal: Cells Date: 2022-09-20 Impact factor: 7.666
Authors: Edward Hannon; Marco Pellegrini; Federico Scottoni; Natalie Durkin; Soichi Shibuya; Roberto Lutman; Toby J Proctor; J Ciaran Hutchinson; Owen J Arthurs; Demetra-Ellie Phylactopoulos; Elizabeth F Maughan; Colin R Butler; Simon Eaton; Mark W Lowdell; Paola Bonfanti; Luca Urbani; Paolo De Coppi Journal: iScience Date: 2022-09-22