Laura C Ornelas1, Kalynn Van Voorhies1, Joyce Besheer2,3. 1. Bowles Center for Alcohol Studies, Chapel Hill, NC, USA. 2. Bowles Center for Alcohol Studies, Chapel Hill, NC, USA. joyce_besheer@med.unc.edu. 3. Department of Psychiatry, University of North Carolina At Chapel Hill, Chapel Hill, NC, 27599-7171, USA. joyce_besheer@med.unc.edu.
Abstract
RATIONALE: Experiencing intrusive distressing memories of a traumatic event(s) is a prominent symptom profile for post-traumatic stress disorder (PTSD). Understanding the neurobiological mechanisms associated with this symptom profile can be invaluable for effective treatment for PTSD. OBJECTIVES: Here, we investigated the functional role of the nucleus reuniens (RE), a midline thalamic in modulating stressor-related memory. METHODS: Female Long Evans rats were implanted with a cannula aimed at the RE. The RE was pharmacologically inactivated via muscimol (0.5 mM) prior to exposure to the predator odor stressor trimethylthiazoline (TMT; synthetically derived fox feces component) or water (controls) in a distinct context with bedding material (experiment 1) or no bedding (experiment 2). To measure context reactivity, the index of the contextual memory, 2 weeks following exposure to TMT, rats were re-exposed to the TMT-paired context (in the absence of TMT). RESULTS: In experiment 1, during context re-exposure (with bedding), inactivation of the RE had no effect on context reactivity. In experiment 2, during context re-exposure (no bedding), rats previously exposed to TMT showed decreased immobility compared to controls, indicating reactivity to the context and likely related to theincreased exploration of the environment. Rats in the TMT group that received RE inactivation showed increased immobility relative to rats that received aCSF, suggesting that muscimol pre-treatment blunted context reactivity. CONCLUSION: In conclusion, recruitment of the RE in stressor-related contextual memory appears to be dependent on the contextual environment and whether the animal is able to engage in different stress coping strategies.
RATIONALE: Experiencing intrusive distressing memories of a traumatic event(s) is a prominent symptom profile for post-traumatic stress disorder (PTSD). Understanding the neurobiological mechanisms associated with this symptom profile can be invaluable for effective treatment for PTSD. OBJECTIVES: Here, we investigated the functional role of the nucleus reuniens (RE), a midline thalamic in modulating stressor-related memory. METHODS: Female Long Evans rats were implanted with a cannula aimed at the RE. The RE was pharmacologically inactivated via muscimol (0.5 mM) prior to exposure to the predator odor stressor trimethylthiazoline (TMT; synthetically derived fox feces component) or water (controls) in a distinct context with bedding material (experiment 1) or no bedding (experiment 2). To measure context reactivity, the index of the contextual memory, 2 weeks following exposure to TMT, rats were re-exposed to the TMT-paired context (in the absence of TMT). RESULTS: In experiment 1, during context re-exposure (with bedding), inactivation of the RE had no effect on context reactivity. In experiment 2, during context re-exposure (no bedding), rats previously exposed to TMT showed decreased immobility compared to controls, indicating reactivity to the context and likely related to theincreased exploration of the environment. Rats in the TMT group that received RE inactivation showed increased immobility relative to rats that received aCSF, suggesting that muscimol pre-treatment blunted context reactivity. CONCLUSION: In conclusion, recruitment of the RE in stressor-related contextual memory appears to be dependent on the contextual environment and whether the animal is able to engage in different stress coping strategies.
Authors: Lara S Hwa; Sofia Neira; Melanie M Pina; Dipanwita Pati; Rachel Calloway; Thomas L Kash Journal: Neuropsychopharmacology Date: 2018-11-23 Impact factor: 7.853
Authors: Maanasa Jayachandran; Stephanie B Linley; Maximilian Schlecht; Stephen V Mahler; Robert P Vertes; Timothy A Allen Journal: Cell Rep Date: 2019-07-16 Impact factor: 9.423