Aggressive corrosion of carbon steel by Desulfovibrio ferrophilus IS5 biofilm was further accelerated by riboflavin.

EET (extracellular electron transfer) is behind MIC (microbiologically influenced corrosion) of carbon steel by SRB (sulfate reducing bacteria). This work evaluated 20 ppm (w/w) riboflavin (an electron mediator) acceleration of C1018 carbon steel MIC by Desulfovibrio ferrophilus IS5 in enriched artificial seawater (EASW) after 7-d incubation in anaerobic vials at 28 °C. Twenty ppm riboflavin did not significantly change cell growth or alter the corrosion product varieties, but it led to 52% increase in weight loss and 105% increase in pit depth, compared to the control without 20 ppm riboflavin. With 20 ppm riboflavin supplement in EASW, D. ferrophilus yielded weight loss-based corrosion rate of 1.57 mm/y (61.8 mpy), and pit depth growth rate of 2.88 mm/y (113 mpy), highest reported for short-term pure-strain SRB MIC of carbon steel. Electrochemical tests in 450 mL glass cells indicated that the biofilm responded rather quickly to the riboflavin injection (20 ppm in broth) to the culture medium. Polarization resistance (Rp) began to decrease within minutes after injection. Within 2 h, the riboflavin injection led to 31% decrease in Rp and 35% decrease in Rct + Rf from electrochemical impedance spectroscopy (EIS). The Tafel corrosion current density increased 63% 2 h after the injection.