Moupriya Nag1, Dibyajit Lahiri1, Sayantani Garai1, Dipro Mukherjee1, Rina Rani Ray2. 1. Department of Biotechnology, University of Engineering & Management, Kolkata, India. 2. Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, West Bengal, India. raypumicro@gmail.com.
Abstract
BACKGROUND: The major activity of β-amylase (BMY) is the production of maltose by the hydrolytic degradation of starch. BMY is found to be produced by some plants and few microorganisms only. The industrial importance of the enzyme warrants its application in a larger scale with the help of genetic engineering, for which the regulatory mechanism is to be clearly understood. RESULTS AND CONCLUSION: In plants, the activities of BMY are regulated by various environmental stimuli including stress of drought, cold and heat. In vascular plant, Arabidopsis sp. the enzyme is coded by nine BAM genes, whereas in most bacteria, BMY enzymes are coded by the spoII gene family. The activities of these genes are in turn controlled by various compounds. Production and inhibition of the microbial BMY is regulated by the activation and inactivation of various BAM genes. Various types of transcriptional regulators associated with the plant- BMYs regulate the production of BMY enzyme. The enhancement in the expression of such genes reflects evolutionary significance. Bacterial genes, on the other hand, as exemplified by Bacillus sp and Clostridium sp, clearly depict the importance of a single regulatory gene, the absence or mutation of which totally abolishes the BMY activity.
BACKGROUND: The major activity of β-amylase (BMY) is the production of maltose by the hydrolytic degradation of starch. BMY is found to be produced by some plants and few microorganisms only. The industrial importance of the enzyme warrants its application in a larger scale with the help of genetic engineering, for which the regulatory mechanism is to be clearly understood. RESULTS AND CONCLUSION: In plants, the activities of BMY are regulated by various environmental stimuli including stress of drought, cold and heat. In vascular plant, Arabidopsis sp. the enzyme is coded by nine BAM genes, whereas in most bacteria, BMY enzymes are coded by the spoII gene family. The activities of these genes are in turn controlled by various compounds. Production and inhibition of the microbial BMY is regulated by the activation and inactivation of various BAM genes. Various types of transcriptional regulators associated with the plant- BMYs regulate the production of BMY enzyme. The enhancement in the expression of such genes reflects evolutionary significance. Bacterial genes, on the other hand, as exemplified by Bacillus sp and Clostridium sp, clearly depict the importance of a single regulatory gene, the absence or mutation of which totally abolishes the BMY activity.
Authors: S Vajravijayan; S Pletnev; N Mani; N Pletneva; N Nandhagopal; K Gunasekaran Journal: Int J Biol Macromol Date: 2018-02-23 Impact factor: 6.953
Authors: Subash C B Gopinath; Periasamy Anbu; M K Md Arshad; Thangavel Lakshmipriya; Chun Hong Voon; Uda Hashim; Suresh V Chinni Journal: Biomed Res Int Date: 2017-02-09 Impact factor: 3.411