Enzymatic recognition of hydrogen peroxide (H2 O2 ) in human plasma samples using HRP immobilized on the surface of poly(arginine-toluidine blue)- Fe3 O4 nanoparticles modified polydopamine; A novel biosensor.

In this study, an innovative strategy was proposed for the electrocatalytical reduction and enzymatic biosensing of hydrogen peroxide (H2 O2 ) using chronoamperometry technique. For the first time, immobilization of horseradish peroxidase (HRP) in polydopamine-modified magnetic nanoparticles (PDA-MNPs) was successfully performed. Also, poly(l-arginine/toluidine blue) film-modified glassy carbon electrode was constructed through co-electropolymerization of l-arginine and toluidine blue on the surface of GCE using cyclic voltammetry technique. The engineered hybrid thin film provides strong functionalities for efficient grafting of PDA-MNPs which, in turn, enable the covalent immobilization of HRP. The proposed biosensor was used for the detection of H2 O2 in the range of 0.5-30 μM with a low limit of quantification 0.23 μM. It also was successfully applied for the investigation of hydrogen peroxide in human plasma samples.