Preparation of projection-less particles from influenza virus and their messenger activities in prokaryotic and eukaryotic systems.

A fraction of projection-less particles was prepared from influenza A/Dunedin/4/73 and A/Victoria/3/75 (X-47) (H3N2) by detergent treatment and extraction into ether at 0 degrees C. The activity of this material in stimulating protein synthesis in vitro was studied and compared with that of isolated virion RNA using a) an RNA-dependent E. coli system, and b) a wheat germ system. In the bacterial system the purified RNA had the highest template activity, while in the eukaryotic system the disrupted particle preparation was by far the most active. Translation products were formed with immunological and electrophoretic properties similar to those of several influenza virion proteins. The experiments indicate that, when added in the form of disrupted projection-less particles, RNA from influenza A2 virus is utilized as a template by eukaryotic ribosomes.