Fatemeh Bootorabi1, Farshid Saadat2, Reza Falak3, Hamed Manouchehri4, Reza Changizi4, Hasan Mohammadi5, Farnaz Safavifar6, Mohammad Reza Khorramizadeh7,8. 1. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Endocrinology and Metabolism Research Institute (EMRI), Next to Dr. Shariati Hospital,#10 Jalal Al-E-Ahmad Expy, 1411713119, Tehran, Iran. 2. Department of Immunology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. 3. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. 4. Department of Aquaculture, Babol Branch of Islamic Azad University, 47134, Babol, Iran. 5. Zebrafish Core Facility, Endocrinology and Metabolism Research Institute (EMRI), Tehran University of Medical Sciences, Tehran, Iran. 6. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. 7. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Endocrinology and Metabolism Research Institute (EMRI), Next to Dr. Shariati Hospital,#10 Jalal Al-E-Ahmad Expy, 1411713119, Tehran, Iran. khoramza@tums.ac.ir. 8. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. khoramza@tums.ac.ir.
Abstract
OBJECTIVE: Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient's circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology. RESULTS: In this trial 3-month old male adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After 5 days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p = 0.0001). These results suggest that probiotic intervention decreased the blood glucose level in the T2DM-P group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.
OBJECTIVE:Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient's circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology. RESULTS: In this trial 3-month old male adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After 5 days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p = 0.0001). These results suggest that probiotic intervention decreased the blood glucose level in the T2DM-P group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.
Authors: Hadi Atabati; Seyed-Alireza Esmaeili; Ehsan Saburi; Maedeh Akhlaghi; Amir Raoofi; Nima Rezaei; Amir Abbas Momtazi-Borojeni Journal: J Cell Physiol Date: 2020-04-28 Impact factor: 6.384