| Literature DB >> 34370167 |
N Berríos-Cartagena1, M M Rubio-Dávila1, I Rivera-Delgado1, M M Feliciano-Bonilla1, E A De Cardona-Juliá1, J G Ortiz2.
Abstract
Glutamate (Glu) is considered the most important excitatory amino acid neurotransmitter in the mammalian Central Nervous System. Zinc (Zn) is co-released with Glu during synaptic transmission and interacts with Glutamate receptors and transporters. We performed binding experiments using [3H]MK-801 (NMDA), and [3H]Fluorowillardine (AMPA) as ligands to study Zn-Glutamate interactions in rat cortical synaptic membranes. We also examined the effects of mercury and lead on NMDA or AMPA receptors. Zinc at 1 nM, significantly potentiates [3H]MK-801 binding. Lead inhibits [3H]MK-801 binding at micromolar concentrations. At millimolar concentrations, Hg also has a significant inhibitory effect. These effects are not reversed by Zn (1 nM). Zinc displaces the [3H]FW binding curve to the right. Lead (nM) and Hg (μM) inhibit [3H]FW binding. At certain concentrations, Zn reverses the effects of these metals on [3H]FW binding. These specific interactions serve to clarify the role of Zn, Hg, and Pb in physiological and pathological conditions.Entities:
Keywords: Lead; Mercury; Zinc; [3H]MK-801; [3H]flurowillardiine
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Year: 2021 PMID： 34370167 PMCID： PMC8672795 DOI： 10.1007/s11064-021-03407-w
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996