BACKGROUND: SARS-CoV-2 pandemic is currently ongoing, meanwhile vaccinations are rapidly underway in some countries. The quantitative immunoassays detecting antibodies against spike antigen of SARS-CoV-2 have been developed based on the findings that they have a better correlation with the neutralizing antibody. METHODS: The performances of the Abbott Architect SARS-CoV-2 IgG II Quant, DiaSorin LIAISON SARS-CoV-2 TrimericS IgG, and Roche Elecsys anti-SARS-CoV-2 S were evaluated on 173 sera from 126 SARS-CoV-2 patients and 151 pre-pandemic sera. Their correlations with GenScript cPass SARS-CoV-2 Neutralization Antibody Detection Kit were also analyzed on 173 sera from 126 SARS-CoV-2 patients. RESULTS: Architect SARS-CoV-2 IgG II Quant and Elecsys anti-SARS-CoV-2 S showed the highest overall sensitivity (96.0%), followed by LIAISON SARS-CoV-2 TrimericS IgG (93.6%). The specificities of Elecsys anti-SARS-CoV-2 S and LIAISON SARS-CoV-2 TrimericS IgG were 100.0%, followed by Architect SARS-CoV-2 IgG II Quant (99.3%). Regarding the correlation with cPass neutralization antibody assay, LIAISON SARS-CoV-2 TrimericS IgG showed the best correlation (Spearman rho = 0.88), followed by Architect SARS-CoV-2 IgG II Quant and Elecsys anti-SARS-CoV-2 S (all rho = 0.87). CONCLUSIONS: The three automated quantitative immunoassays showed good diagnostic performance and strong correlations with neutralization antibodies. These assays will be useful in diagnostic assistance, evaluating the response to vaccination, and the assessment of herd immunity in the future.
BACKGROUND:SARS-CoV-2 pandemic is currently ongoing, meanwhile vaccinations are rapidly underway in some countries. The quantitative immunoassays detecting antibodies against spike antigen of SARS-CoV-2 have been developed based on the findings that they have a better correlation with the neutralizing antibody. METHODS: The performances of the Abbott Architect SARS-CoV-2 IgG II Quant, DiaSorin LIAISON SARS-CoV-2 TrimericS IgG, and Roche Elecsys anti-SARS-CoV-2 S were evaluated on 173 sera from 126 SARS-CoV-2patients and 151 pre-pandemic sera. Their correlations with GenScript cPassSARS-CoV-2 Neutralization Antibody Detection Kit were also analyzed on 173 sera from 126 SARS-CoV-2patients. RESULTS: Architect SARS-CoV-2 IgG II Quant and Elecsys anti-SARS-CoV-2 S showed the highest overall sensitivity (96.0%), followed by LIAISON SARS-CoV-2 TrimericS IgG (93.6%). The specificities of Elecsys anti-SARS-CoV-2 S and LIAISON SARS-CoV-2 TrimericS IgG were 100.0%, followed by Architect SARS-CoV-2 IgG II Quant (99.3%). Regarding the correlation with cPass neutralization antibody assay, LIAISON SARS-CoV-2 TrimericS IgG showed the best correlation (Spearman rho = 0.88), followed by Architect SARS-CoV-2 IgG II Quant and Elecsys anti-SARS-CoV-2 S (all rho = 0.87). CONCLUSIONS: The three automated quantitative immunoassays showed good diagnostic performance and strong correlations with neutralization antibodies. These assays will be useful in diagnostic assistance, evaluating the response to vaccination, and the assessment of herd immunity in the future.
Authors: Victoria Furer; Tali Eviatar; Devy Zisman; Hagit Peleg; Yolanda Braun-Moscovici; Alexandra Balbir-Gurman; Daphna Paran; David Levartovsky; Michael Zisapel; Ofir Elalouf; Ilana Kaufman; Adi Broyde; Ari Polachek; Joy Feld; Amir Haddad; Tal Gazitt; Muna Elias; Nizar Higazi; Fadi Kharouf; Sara Pel; Sharon Nevo; Ori Elkayam Journal: Vaccines (Basel) Date: 2022-06-06
Authors: Jakub Swadźba; Tomasz Anyszek; Andrzej Panek; Agnieszka Chojęta; Kinga Wyrzykowska; Emilia Martin Journal: Diagnostics (Basel) Date: 2022-06-09
Authors: Georgina Ireland; Heather Whitaker; Shamez N Ladhani; Frances Baawuah; Sathyvani Subbarao; Ezra Linley; Lenesha Warrener; Michelle O'Brien; Corinne Whillock; Oliver Martin; Paul Moss; Mary E Ramsay; Gayatri Amirthalingam; Kevin E Brown Journal: Euro Surveill Date: 2022-01
Authors: Max Schuller; Verena Pfeifer; Alexander H Kirsch; Konstantin A Klötzer; Agnes A Mooslechner; Alexander R Rosenkranz; Philipp Stiegler; Peter Schemmer; Harald Sourij; Philipp Eller; Barbara Prietl; Kathrin Eller Journal: Front Med (Lausanne) Date: 2022-02-02