SILO: A Computational Method for Detecting Copy Number Gain in Clinical Specimens Analyzed on a Next-Generation Sequencing Platform.

Next-generation sequencing (NGS) has proved to be a beneficial approach for genotyping solid tumor specimens and for identifying clinically actionable mutations. However, copy number variations (CNVs), which can be equally important, are often challenging to detect from NGS data. Current bioinformatics methods for CNV detection from NGS often require comparison of tumor/normal pairs and/or the sequencing of whole genome or whole exome. These approaches are currently impractical for routine clinical practice. However, clinical practice does involve repeated use of the same gene panel on a large number of specimens over a long period of time. We take advantage of this repetitiveness and present SILO: a procedure for CNV detection based on NGS on a gene panel. The SILO algorithm analyzes coverage depth of the aligned reads from a sample and predicts CNV by comparing this depth to the average depth seen in a large training set of other samples. Such comparison is robust and can reliably detect copy number gain, although it is found to be unreliable in detecting copy number losses. Successful validation of SILO on NGS data from the Ion Torrent platform with two panels is presented: a small hotspot panel and a larger cancer gene panel.