Keltoum Alliouche Kerboua1, Lilia Benosmane2, Selma Namoune2, Khaoula Ouled-Diaf2, Nora Ghaliaoui3, Dalila Bendjeddou2. 1. Laboratoire Biologie, Eau et Environnement (LBEE), Université 8 Mai 1945 Guelma, BP, 401, Guelma, 24000, Algeria. Electronic address: keltoum.alliouchekerboua@gmail.com. 2. Faculty of Natural and Life Sciences - Sciences of the Earth and the Universe, University of 8 Mai 1945, Guelma, Algeria. 3. Center of Research in Physical and Chemical Analysis CRAPC, BP 248 Algiers, RP, 16004, Algiers, Algeria.
Abstract
ETHNOPHAMACOLOGICAL RELEVANCE: the roots of Anacyclus pyrethrum (L.) Lag. (Family: Asteraceae) are used in Algeria to treat respiratory infections, to cure chronic head and nostrils catarrh, and to clear the brain by stimulating the free flow of nasal mucous. They contain a high quantity of hot water-soluble polysaccharides. AIMS OF THE STUDY: The study aims to evaluate the antioxidant and anti-inflammatory activity of polysaccharides extracted from Anacyclus pyrethrum roots (APPS). MATERIALS AND METHODS: The APPS were extracted using boiling water, separated from proteins by the Sevag method then precipitated with 90% ethanol. The antioxidant effect of crude APPS was evaluated using FRAP assay. To investigate the anti-inflammatory potential, mice were treated with crude polysaccharides (25, 50, and 100 mg/kg, i.p.) for 3 days (14th, 15th, and 16th day of the experimentation). Respiratory inflammation was induced by HDM (House Dust Mite), mice were sensitized intranasally with 25 μg HDM suspended in 10 μl NaCl (5 μl/nostril) on days 0 and 7 then challenged with 5 μg HDM on days 14, 15, and 16. Mice were sacrificed 24 h after the last challenge. The number of immune cells in the blood in NL (Nasal Liquid) and in BAL (Broncho Alveolar Liquid) was enumerated, the spleen was removed to calculate the relative spleen weight and to count splénocytes, lungs histopathological examination was carried out to confirm the protective effect of APPS. Structural characterization of APPS was identified using FTIR (Fourier-Transform Infrared Spectroscopy) and SEM (Scanning Electron Microscopy). RESULTS: The crude APPS possessed reducing power. In vivo assay, treatment with APPS causes a decrease in the number of blood leucocytes at all doses on the one hand, and in the relative spleen weight and splénocytes number on the other hand except at the dose of 50 mg/kg in which an enhancement of the number of splénocytes and immune cells in NL and BAL was significant. The histopathological examination showed clear protection of lung tissue damaged by HDM, after treatment with APPS mainly, at the dose of 50 mg/kg. CONCLUSION: Our data clearly showed antioxidant and anti-inflammatory activity of APPS on HDM-challenged mice induced lungs inflammation by equilibrating the inflammatory reaction mostly, with an optimal dose of 50 mg/kg.
ETHNOPHAMACOLOGICAL RELEVANCE: the roots of Anacyclus pyrethrum (L.) Lag. (Family: Asteraceae) are used in Algeria to treat respiratory infections, to cure chronic head and nostrils catarrh, and to clear the brain by stimulating the free flow of nasal mucous. They contain a high quantity of hot water-soluble polysaccharides. AIMS OF THE STUDY: The study aims to evaluate the antioxidant and anti-inflammatory activity of polysaccharides extracted from Anacyclus pyrethrum roots (APPS). MATERIALS AND METHODS: The APPS were extracted using boiling water, separated from proteins by the Sevag method then precipitated with 90% ethanol. The antioxidant effect of crude APPS was evaluated using FRAP assay. To investigate the anti-inflammatory potential, mice were treated with crude polysaccharides (25, 50, and 100 mg/kg, i.p.) for 3 days (14th, 15th, and 16th day of the experimentation). Respiratory inflammation was induced by HDM (House Dust Mite), mice were sensitized intranasally with 25 μg HDM suspended in 10 μl NaCl (5 μl/nostril) on days 0 and 7 then challenged with 5 μg HDM on days 14, 15, and 16. Mice were sacrificed 24 h after the last challenge. The number of immune cells in the blood in NL (Nasal Liquid) and in BAL (Broncho Alveolar Liquid) was enumerated, the spleen was removed to calculate the relative spleen weight and to count splénocytes, lungs histopathological examination was carried out to confirm the protective effect of APPS. Structural characterization of APPS was identified using FTIR (Fourier-Transform Infrared Spectroscopy) and SEM (Scanning Electron Microscopy). RESULTS: The crude APPS possessed reducing power. In vivo assay, treatment with APPS causes a decrease in the number of blood leucocytes at all doses on the one hand, and in the relative spleen weight and splénocytes number on the other hand except at the dose of 50 mg/kg in which an enhancement of the number of splénocytes and immune cells in NL and BAL was significant. The histopathological examination showed clear protection of lung tissue damaged by HDM, after treatment with APPS mainly, at the dose of 50 mg/kg. CONCLUSION: Our data clearly showed antioxidant and anti-inflammatory activity of APPS on HDM-challenged mice induced lungs inflammation by equilibrating the inflammatory reaction mostly, with an optimal dose of 50 mg/kg.
Authors: Saida Sissi; Silvia Di Giacomo; Claudio Ferrante; Paola Angelini; Alberto Macone; Anna Maria Giusti; Chiara Toniolo; Annabella Vitalone; Aghraz Abdellah; Mustapha Larhsini; Luigi Menghini; Mohammed Markouk; Gabriela Mazzanti; Antonella Di Sotto Journal: Molecules Date: 2022-01-21 Impact factor: 4.411