Liyuan Zheng1, Ke Li2, Congqin Ning3, Jian Sun4. 1. Department of prosthodontics, School of stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of medicine, National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai 200011, China. 2. State Key Laboratory of High Performance Ceramics and Super fine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. 3. State Key Laboratory of High Performance Ceramics and Super fine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234, China. Electronic address: cqning@mail.sic.ac.cn. 4. Department of prosthodontics, School of stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of medicine, National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai 200011, China. Electronic address: doctorsunjian74@163.com.
Abstract
OBJECTIVE: A novel composite resin (CR) with fluorine-doped nano-zirconia (F-ZrO2) fillers was developed as an antibacterial restorative material. This article described the synthesis and investigated the fluoride release, antibacterial property and cytotoxicity of the novel CR. METHODS: F-ZrO2 powders with different F fluorine contents (0% F-ZrO2, 5% F-ZrO2, 10% F-ZrO2 or 20% F-ZrO2) were synthesized by chemical precipitation method and characterized by XRD, SEM and TEM-EDS. The content and release of fluoride were also determined. 20% F-ZrO2 powers were selected to develop the novel CRs (FZ-25, FZ-50, and FZ-75). The fluoride release from the novel CRs during 28 days was recorded. The antibacterial property of the novel CRs was investigated with direct contact test (DCT) and metabolic activity test (CCK8). The cytotoxicity of the CRs was also evaluated here. RESULTS: F-ZrO2 powders with different F fluorine contents were obtained. The fluoride release increased with increasing of the fluoride content. Through the antibacterial performance evaluation, 20% F-ZrO2 powders, which exhibiting the best antibacterial property, were selected as the fillers for preparing the novel CR. The novel CR with F-ZrO2 fillers exhibited an effective antibacterial effect. Compared with the control group, the antibacterial rates of FZ-25, FZ-50 and FZ-75 were 51.65%, 54.14% and 66.80% (p<0.05), respectively. No obvious cytotoxicity of the novel CR was detected in this study. CLINICAL SIGNIFICANCE: The novel CR with continuous fluoride release and proper antibacterial property is expected to be used as an antibacterial material to reduce secondary caries.
OBJECTIVE: A novel composite resin (CR) with fluorine-doped nano-zirconia (F-ZrO2) fillers was developed as an antibacterial restorative material. This article described the synthesis and investigated the fluoride release, antibacterial property and cytotoxicity of the novel CR. METHODS:F-ZrO2 powders with different Ffluorine contents (0% F-ZrO2, 5% F-ZrO2, 10% F-ZrO2 or 20% F-ZrO2) were synthesized by chemical precipitation method and characterized by XRD, SEM and TEM-EDS. The content and release of fluoride were also determined. 20% F-ZrO2 powers were selected to develop the novel CRs (FZ-25, FZ-50, and FZ-75). The fluoride release from the novel CRs during 28 days was recorded. The antibacterial property of the novel CRs was investigated with direct contact test (DCT) and metabolic activity test (CCK8). The cytotoxicity of the CRs was also evaluated here. RESULTS:F-ZrO2 powders with different Ffluorine contents were obtained. The fluoride release increased with increasing of the fluoride content. Through the antibacterial performance evaluation, 20% F-ZrO2 powders, which exhibiting the best antibacterial property, were selected as the fillers for preparing the novel CR. The novel CR with F-ZrO2 fillers exhibited an effective antibacterial effect. Compared with the control group, the antibacterial rates of FZ-25, FZ-50 and FZ-75 were 51.65%, 54.14% and 66.80% (p<0.05), respectively. No obvious cytotoxicity of the novel CR was detected in this study. CLINICAL SIGNIFICANCE: The novel CR with continuous fluoride release and proper antibacterial property is expected to be used as an antibacterial material to reduce secondary caries.