Literature DB >> 3436053

A new micro-assay for human liver alanine: glyoxylate aminotransferase.

J Allsop1, P R Jennings, C J Danpure.   

Abstract

A micro radiochemical method has been developed for the assay of the human liver peroxisomal enzyme alanine: glyoxylate aminotransferase (EC 2.6.1.44). The method, based on the electrophoretic separation of [14C]alanine (substrate) from [14C]pyruvate (product) is at least fifty times more sensitive than the currently-used spectrophotometric double enzyme method (Rowsell et al, Int J Biochem 1972;3: 247-257), enabling the enzymatic diagnosis of primary hyperoxaluria type 1 to be carried out on only 100 micrograms of human liver tissue obtained by percutaneous needle biopsy. The increased sensitivity of the new method allows the assay conditions to be such that they are on the linear parts of the time-course and protein concentration curves. This results in the activities of alanine: glyoxylate aminotransferase in human liver samples being 20-50% higher than those determined by the spectrophotometric method.

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Year:  1987        PMID： 3436053     DOI： 10.1016/0009-8981(87)90127-6

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  10 in total

Review 1.  Recent advances in the understanding, diagnosis and treatment of primary hyperoxaluria type 1.

Authors:  C J Danpure
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

2.  Enzymatic prenatal diagnosis of primary hyperoxaluria type 1: potential and limitations.

Authors:  C J Danpure; P J Cooper; P R Jennings; P J Wise; R J Penketh; C H Rodeck
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

3.  Enzymatic heterogeneity in primary hyperoxaluria type 1 (hepatic peroxisomal alanine: glyoxylate aminotransferase deficiency).

Authors:  C J Danpure; P R Jennings
Journal:  J Inherit Metab Dis       Date:  1988       Impact factor: 4.982

4.  Rapid identification of primary hyperoxaluria type I patients using a novel, fully automated method for measurement of hepatic alanine: glyoxylate aminotransferase.

Authors:  V A Horváth; R J Wanders
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

5.  [Type I oxalosis in childhood--studies within the scope of terminal renal failure in the child].

Authors:  M Frosch; E Kuwertz-Bröking; M Bulla; D B von Bassewitz; D B Leusmann
Journal:  Klin Wochenschr       Date:  1989-11-17

6.  Molecular characterization and clinical use of a polymorphic tandem repeat in an intron of the human alanine:glyoxylate aminotransferase gene.

Authors:  C J Danpure; G M Birdsey; G Rumsby; M J Lumb; P E Purdue; J Allsop
Journal:  Hum Genet       Date:  1994-07       Impact factor: 4.132

Review 7.  Primary hyperoxaluria type 1: genotypic and phenotypic heterogeneity.

Authors:  C J Danpure; P R Jennings; P Fryer; P E Purdue; J Allsop
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

8.  Enzymological and mutational analysis of a complex primary hyperoxaluria type 1 phenotype involving alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting and intraperoxisomal aggregation.

Authors:  C J Danpure; P E Purdue; P Fryer; S Griffiths; J Allsop; M J Lumb; K M Guttridge; P R Jennings; J I Scheinman; S M Mauer
Journal:  Am J Hum Genet       Date:  1993-08       Impact factor: 11.025

9.  Enzymological characterization of a feline analogue of primary hyperoxaluria type 2: a model for the human disease.

Authors:  C J Danpure; P R Jennings; J Mistry; R A Chalmers; R E McKerrell; W F Blakemore; M F Heath
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

10.  An enzyme trafficking defect in two patients with primary hyperoxaluria type 1: peroxisomal alanine/glyoxylate aminotransferase rerouted to mitochondria.

Authors:  C J Danpure; P J Cooper; P J Wise; P R Jennings
Journal:  J Cell Biol       Date:  1989-04       Impact factor: 10.539
  10 in total

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