Roland Bocco1,2, Moran Lee1, Dayeon Kim1, Seongho Ahn1, Jin-Woo Park1, Sang-Yeob Lee3, Ji-Hee Han4. 1. Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA, Wanju 565-851, Korea. 2. Department of Agriculture and Natural Resources (ANR), UC Davis, Cooperative Extension, 2156, Sierra Way, Ste C, San Luis Obispo, CA 93401, USA. 3. The Korea Agro-Materials Research Organization (KARO), 703, Farmer's Building 92, Suseong-ro, Paldal-gu, Suwon-si 16432, Korea. 4. Planning and Coordination Division, National Institute of Agricultural Sciences, RDA, Wanju 565-851, Korea.
Abstract
This study endeavored to sustainably control aphids and anthracnose after spraying endophytic Isaria javanica pf185 under field conditions. Under two different tents; one batch of seedlings was sprayed with a 107 conidia/mL I. javanica pf185 suspension; while another was sprayed with 0.05% Tween 80® in distilled water. Six leaf discs from the top; middle; and bottom part of the plant canopy were weekly collected and placed on moistened filter paper in a Petri dish for insecticidal and antifungal bioassays against Myzus persicae and Colletotrichum acutatum. Differences were noticed from the 18th day after spraying with mortality (86.67 ± 0.57% versus 36.67 ± 0.64%) and leaf damage (13.45 ± 0.03% versus 41.18 ± 0.06%) on fungus-treated and controlled, respectively. The corrected insecticidal efficacy was 20.43, 39.82, 72.32, 66.43 and 70.04%, while the corrected fungicidal efficacy was 26.07, 38.01, 53.35, 29.08 and 41.81% during five successive weeks. A positive correlation was evident between insecticidal efficacy and relative humidity (r2 = 0.620) and temperature (r2 = 0.424), respectively. No correlation was found between antifungal activity and relative humidity (r2 = 0.061) and temperature (r2 = 0), respectively. The entomopathogenic fungus survived on leaf surface area and in tissues after spraying.
This study endeavored to sustainably control aphids and anthracnose after spn>raying endopn>hytic n>an class="Species">Isaria javanica pf185 under field conditions. Under two different tents; one batch of seedlings was sprayed with a 107 conidia/mL I. javanica pf185 suspension; while another was sprayed with 0.05% Tween 80® in distilledwater. Six leaf discs from the top; middle; and bottom part of the plant canopy were weekly collected and placed on moistened filter paper in a Petri dish for insecticidal and antifungal bioassays against Myzus persicae and Colletotrichum acutatum. Differences were noticed from the 18th day after spraying with mortality (86.67 ± 0.57% versus 36.67 ± 0.64%) and leaf damage (13.45 ± 0.03% versus 41.18 ± 0.06%) on fungus-treated and controlled, respectively. The corrected insecticidal efficacy was 20.43, 39.82, 72.32, 66.43 and 70.04%, while the corrected fungicidal efficacy was 26.07, 38.01, 53.35, 29.08 and 41.81% during five successive weeks. A positive correlation was evident between insecticidal efficacy and relative humidity (r2 = 0.620) and temperature (r2 = 0.424), respectively. No correlation was found between antifungal activity and relative humidity (r2 = 0.061) and temperature (r2 = 0), respectively. The entomopathogenic fungus survived on leaf surface area and in tissues after spraying.
Authors: Mustapha F A Jallow; Dawood G Awadh; Mohammed S Albaho; Vimala Y Devi; Binson M Thomas Journal: Sci Total Environ Date: 2016-10-14 Impact factor: 7.963
Authors: B A Bailey; H Bae; M D Strem; D P Roberts; S E Thomas; J Crozier; G J Samuels; Ik-Young Choi; K A Holmes Journal: Planta Date: 2006-07-11 Impact factor: 4.116