Literature DB >> 3435469

Measurement of the matrix free Ca2+ concentration in heart mitochondria by entrapped fura-2 and quin2.

G L Lukács1, A Kapus.   

Abstract

A method was developed to monitor continuously the matrix free Ca2+ concentration ([Ca2+]m) of heart mitochondria by use of the fluorescent Ca2+ indicators, fura-2 and quin2. The acetoxymethyl esters of fura-2 and quin2 were accumulated in and hydrolysed by isolated mitochondria. An increase of the mitochondrial Ca content from 0.3 nmol/mg of protein to 6 nmol/mg corresponded to a rise of [Ca2+]m from 30 to 1000 nM. The results indicate that physiological fluctuations of the mitochondrial Ca content elicit changes of [Ca2+]m in that range which regulates the matrix dehydrogenases.

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Year:  1987        PMID： 3435469      PMCID： PMC1148585          DOI： 10.1042/bj2480609

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  26 in total

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Journal:  Am J Physiol       Date:  1985-12

2.  Alteration of intracellular [Ca2+] in sea urchin sperm by the egg peptide speract. Evidence that increased intracellular Ca2+ is coupled to Na+ entry and increased intracellular pH.

Authors:  R W Schackmann; P B Chock
Journal:  J Biol Chem       Date:  1986-07-05       Impact factor: 5.157

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Authors:  J S Puskin; T E Gunter; K K Gunter; P R Russell
Journal:  Biochemistry       Date:  1976-08-24       Impact factor: 3.162

4.  Regulation of free and bound magnesium in rat hepatocytes and isolated mitochondria.

Authors:  B E Corkey; J Duszynski; T L Rich; B Matschinsky; J R Williamson
Journal:  J Biol Chem       Date:  1986-02-25       Impact factor: 5.157

5.  The Ba2+ sensitivity of the Na+-induced Ca2+ efflux in heart mitochondria: the site of inhibitory action.

Authors:  G L Lukács; A Fonyó
Journal:  Biochim Biophys Acta       Date:  1986-06-13

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Authors:  C L Moore
Journal:  Biochem Biophys Res Commun       Date:  1971-01-22       Impact factor: 3.575

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Authors:  D A Williams; K E Fogarty; R Y Tsien; F S Fay
Journal:  Nature       Date:  1985 Dec 12-18       Impact factor: 49.962

Review 8.  Relation between mitochondrial calcium transport and control of energy metabolism.

Authors:  R G Hansford
Journal:  Rev Physiol Biochem Pharmacol       Date:  1985       Impact factor: 5.545

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Authors:  R M Denton; J G McCormack
Journal:  FEBS Lett       Date:  1980-09-22       Impact factor: 4.124

10.  Phosphate transport, membrane potential, and movements of calcium in rat liver mitochondria.

Authors:  E Ligeti; G L Lukács
Journal:  J Bioenerg Biomembr       Date:  1984-04       Impact factor: 2.945
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  21 in total

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Authors:  David G Nicholls; Susan Chalmers
Journal:  J Bioenerg Biomembr       Date:  2004-08       Impact factor: 2.945

2.  Mitochondrial participation in the intracellular Ca2+ network.

Authors:  D F Babcock; J Herrington; P C Goodwin; Y B Park; B Hille
Journal:  J Cell Biol       Date:  1997-02-24       Impact factor: 10.539

Review 3.  Dehydrogenase activation by Ca2+ in cells and tissues.

Authors:  R G Hansford
Journal:  J Bioenerg Biomembr       Date:  1991-12       Impact factor: 2.945

4.  Dependence of cardiac mitochondrial pyruvate dehydrogenase activity on intramitochondrial free Ca2+ concentration.

Authors:  R Moreno-Sánchez; R G Hansford
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

5.  Intracellular calcium release is more efficient than calcium influx in stimulating mitochondrial NAD(P)H formation in adrenal glomerulosa cells.

Authors:  T Rohács; K Tory; A Dobos; A Spät
Journal:  Biochem J       Date:  1997-12-01       Impact factor: 3.857

6.  Cytoplasmic Ca2+ signalling and reduction of mitochondrial pyridine nucleotides in adrenal glomerulosa cells in response to K+, angiotensin II and vasopressin.

Authors:  T Rohács; G Nagy; A Spät
Journal:  Biochem J       Date:  1997-03-15       Impact factor: 3.857

Review 7.  Physiological role of mitochondrial Ca2+ transport.

Authors:  R G Hansford
Journal:  J Bioenerg Biomembr       Date:  1994-10       Impact factor: 2.945

8.  The Use of Fura-2 Fluorescence to Monitor the Movement of Free Calcium Ions into the Matrix of Plant Mitochondria (Pisum sativum and Helianthus tuberosus).

Authors:  M. Zottini; D. Zannoni
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

9.  Preservation on calcium homeostasis is involved in mitochondrial protection of Limonium sinense against liver damage in mice.

Authors:  Xin-Hui Tang; Jin Chen; Xiao-Lan Yang; Li-Fang Yan; Jing Gao
Journal:  Pharmacogn Mag       Date:  2010-07       Impact factor: 1.085

10.  EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.

Authors:  Horia Vais; Karthik Mallilankaraman; Don-On Daniel Mak; Henry Hoff; Riley Payne; Jessica E Tanis; J Kevin Foskett
Journal:  Cell Rep       Date:  2016-01-07       Impact factor: 9.423
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