Alkaline conditions can affect the volume and chemical characteristics of dental gutta-percha.

The effects of alkaline pH on the size and surface characteristics of gutta-percha were studied. Forty ProTaper Universal-F2 gutta-percha cones (GPC) were stored individually for 8-weeks. GPC were distributed into 2 groups (n = 20) based on the storage solutions' pH (pH7, pH12). The GPC volume was measured after 1,2,3,4 and 8-weeks using water displacement method and compared to the baseline volume. The surfaces of representative GPCs were analysed using Raman spectroscopy and assessed later using Scanning Electron Microscopy with Energy-Dispersive X-Ray Spectroscopy (EDS). Samples t test was used to compare the change in volume of GPC and a repeated measures Analysis of Variance (ANOVA) was used to compare the effects of storage durations. After 8-weeks, GPCs aged in high-pH exhibited significant drop in their volume (16%) compared with GPCs aged in water (2.5%). The surface of GPCs stored in water or high-pH both demonstrated structural and chemical alterations in comparison with non-aged GPCs.