Complement Inhibition and COVID-19: The Story so Far.

Acute respiratory distress syndrome (ARDS) is the most severe complication of COVID-19, a disease caused by severe acute respiratory syndrome coronavirus (SARS CoV) 2. The mechanisms underlying the progression from asymptomatic disease to pneumonia and ARDS are complex and by far unelucidated. As for bacterial sepsis, the release of damage associated molecular patterns and pathogen associated molecular patterns triggers activation of the complement cascade. Subsequently, overexpressed anaphylatoxins recruit inflammatory cells in the lung and other organs and contribute initiating and amplifying a vicious circle of thromboinflammation causing organs damage and eventually death. Preclinical and observational studies in patients with COVID-19 provided evidence that complement inhibition effectively may attenuate lung and systemic inflammation, restore the coagulation/fibrinolysis balance, improve organs function and eventually may save life. Ongoing Phase 2/3 trials should elucidate the benefit to risk profile of complement inhibitors and may clarify the optimal targets in the complement cascade.

Introduction

Since December 2019, COVID-19, a disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) places a major burden on healthcare and economy systems in most if not all countries. As of May 15th 2021, COVID-19 has affected more than 160 million people and caused about 3.4 million deaths.1 It is now well established that following a phase of viral replication in airway and lung tissues, a deregulated immune response is the hallmark of COVID-19 alike bacterial sepsis.2 SARS-CoV-2 triggers immune responses that may vary quantitatively and qualitatively in-between individuals and overtime in the same individual. The very early – within 24 to 48 hours – response to SARS-CoV-2 involved mainly activation of plasmacytoid predendritic cells with subsequent upregulation of interferon type 1 (IFN-1) pathway3 which in turn may trigger TNF/IL-1β-driven inflammatory response.4 Distinct cytokine profiles may be associated with distinct clinical phenotypes with variable prognosis.5 Contrasting with the heterogeneity in clinical phenotypes, a core immune signature carried by peripheral blood cells is characterized by profound T-cells alteration and selective cytokines upregulation, specific antibodies and minimal alterations in B cells.6 The complement system plays a pivotal role in innate immunity.7–9 In sepsis, overactivation of the complement system may favour pro-coagulation over fibrinolysis and inflammation over immune suppression resulting in a vicious circle of self-amplification of thrombo-inflammation.10 Likewise, a dysregulated complement system may partly account for the progression from mild to severe form of COVID-19.11 This narrative review summarized the current knowledge about the activation and deregulation in the complement system during sepsis and COVID-19 and about modulating the complement cascade as a therapeutic approach.

Evidence That COVID-19 is a Complement Mediated Disease

Physiological Basis

The complement system is composed by heat-labile plasma proteins that cross-talk in a catalytic cascade. After Bordet demonstration of the bacteriolytic activity of the serum alexin,7 Ehrlich introduced the term complement.12 A proportionate activation of the complement system is essential to immune homeostasis whereas overactivation may trigger thromboinflammation and end-organ damages and complement deficiency may cause susceptibility to life-threatening infections, and may precede autoimmune disorders. The complement system has about 40 proteins organized in seven functional components (Table 1). The complement system acts as a sentinel that rapidly and with high specificity detects, traces, targets and eradicates pathogens. Its activation involved the classical, mannose-binding lectin (MBL), and alternative pathways. The classical pathway is activated by direct association of C1q with pathogen surfaces or by binding of C1q to antigen-antibody complexes during an adaptive immune response. The MBL pathway is triggered by binding of MBL to mannose containing carbohydrate structures on bacteria or virus surface. The alternative pathway is activated by binding of spontaneously activated complement C3 protein (C3b fragment) to pathogen’s surface. These three pathways converge to the formation of C3 convertase that stimulates the formation of C3a, C3b, C5a, C5b, C6, C7, C8, and C9. The fragments C5b, C6, C7, C8, and C9 form the membrane attack complex (C5b-9, MAC) triggering bacteria lysis (Figure 1). Opsonins and anaphylatoxins boost pathogens phagocytosis and activate circulating white blood cells.9 The proinflammatory effects are finetuned by counter-balancing inhibitory factors such as inactive C3b.13 Each step of the catalytic cascade is tightly controlled by soluble and cell membrane bound regulatory factors (Table 1). Beyond immunity, the complement system has other targets including stem cells and progenitor cells, nervous system, and metabolic tissues modulating cellular stress, apoptosis and autophagy.9

Table 1

Functional Components of the Complement System

Functional Components	Molecules	Functions
Initiator complement factors	C1q complexMannose binding lectinFicolins	Trigger complement cascade by binding to activated soluble or membrane bound ligands
Enzymatic mediators	C3 convertaseC5 convertase	Induce cleavage or conformational changes of complement components
Opsonins	C3b, C4b (largest fragment resulting from convertase cleavage)	Bind to specific receptors at bacterial cell surfaceStimulate phagocytosis
Anaphylatoxins	C3a, C4a, C5a (smallest fragment resulting from convertase cleavage)	Bind to endothelial cells and increase blood flowTrigger cytokines cascade
Membrane attack complex	Assembly of C5b, C6, C7 and C9	Bind and destroy bacteria membrane with subsequent lysis of bacteria
Complement receptors	Cell surface receptors	Bind to complement proteins
Regulatory complement factors	Soluble:Factor HC4-binding proteinCell membrane:Decay-accelerating factorComplement receptor 2Complement receptor 1Membrane cofactor proteinProteolytic enzymes serine protease factor 1	Prevent overactivation of complement proteins

Figure 1

Four major pathways are involved in complement activation: classical, lectin, alternative, and coagulation pathways. The classical pathway is activated by direct association of C1q with pathogen surfaces or by binding of C1q to antigen-antibody complexes during an adaptive immune response. The mannose binding lectin pathway is triggered by binding of MBL to mannose containing carbohydrate structures on bacteria or virus surface. The alternative pathway is activated by binding of spontaneously activated complement C3 protein (C3b fragment) to pathogen’s surface. These three pathways converge to the formation of C3 convertase that stimulates the formation of C3a, C3b, C5a, C5b, C6, C7, C8, and C9. The fragments C5b, C6, C7, C8, and C9 form the membrane attack complex (C5b-9, MAC) triggering bacteria lysis, while C5a is a strong anaphylatoxin. The fourth activation pathway is through the action of thrombin, which catalysis C5 into C5a and C5b.

Role of Anaphylatoxin C5 in the Physiopathology of Sepsis (Figure 2)

Human C5a is a 74 amino acid glycosylated peptide with anti-parallel α-helical structures that are crosslinked by disulphide bands, making the molecule quite stable, especially in the presence of oxidants. C5a is released from the N terminal region of the α chain of C5 by C5 convertases (C3b2•Bb or C4b•C2a•C3b) induced cleavage of C5 into C5a + C5b. C5b interacts with C6, C7, C8 and C9 to form the membrane attack complex C5b-9 (MAC). The release of C5a can also be triggered by neutral proteases derived from neutrophils or lung macrophages, and by thrombin (factor IIa).

Summary of C5a action in sepsis. After binding to its C5aR and C5L2 receptors, C5a will have different effects on the immune system and different organs. The C5a pathway will lead to the secretion of pro-inflammatory cytokines by macrophages such as IL-6, IL-1 or TNF alpha allowing the recruitment of other immune cells. On PMNs, C5a will first increase the expression of CCR to facilitate their recruitment but will then induce a paralysis of ERK1/2 leading to a decrease in phagocytosis or respiratory burst. C5a-exposed endothelial cells obtain an activated phenotype with adhesion molecule and tissue factor expression that will initiate the coagulation cascade with a risk of DIC. Lymphopenia is a feature of sepsis and is related to increased lymphocyte apoptosis via the C5a pathway. C5a induces apoptosis of adrenomedullary cells, which are responsible for the bulk of endogenous catecholamines, leading to a disequilibrium that favors the development of septic shock. The binding of C5a to its receptors on the surface of the cardiomyocytes will lead to a defect in the repolarization of the cell and an accumulation of calcium in the cytoplasm, resulting in a defect in contractility and relaxation of the cardiomyocytes.

The anaphylatoxin C5a contributes to the systemic overactivation of the proinflammatory cytokines that characterize sepsis. In cecal ligation and puncture (CLP) induced sepsis in mice, C5a induced the migration of interleukin-12+ dendritic cell (IL-12+DC) from the peritoneal cavity to blood stream and lymph nodes contributing to the systemic spreading of inflammation. IL-12+DC are key inducers of interferon (IFN)c+ Th1 and IL-17+ Th17 cells. The pleiotropic activities of IL-12 on natural killer (NK), T and B cells function require to be finetuned for an appropriate immune responses. C5a prevents toll-like receptor (TLR)4-induced synthesis of IL-12, IL-23, and IL-27 via extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K). In addition to its role in dampening TLR4-driven synthesis, C5a also regulates CD40-induced IL-12 production through ERK1/2. IFN regulatory factor 1 (IRF-1) and IFN consensus sequence binding protein (ICSBP; IRF-8) are also as crucial transcription factors down-streaming ERK and PI3K pathways, respectively. These data indicate that C5a modulates both innate (TLR4) and adaptive (CD40) immune responses that drive the production of IL-12 family cytokines. C5a also interferes with CD40/CD40 ligand axis involved in the communication between the antigen presenting cell (mostly IL-12+DC) and T cell preventing Th1 polarization.14,15 C5a/C5aR axis interacts synergistically with TLRs stimulating NK cells production of TNF-α and IFN-γ.16 C5a inhibits LPS-induced production of TNF-α in neutrophils and by contrast enhances TNF-α release by macrophages.17 There is also evidence for a synergistic relationship between C5a and LPS to produce neutrophil-derived antigenic IL-8.18 The release of IL-6 is closely linked to up-regulation of C5aR and activation of ERK1/2 and p38 as a final common step involving activation of mitogen and stress activated kinase 1/2 (MSK1/2).19,20 Interestingly, C5a exhibited anti-inflammatory effects by downregulating IL-17A and IL-23 via inhibition of CD11b(+)F4/80(+) macrophages and upregulating IL-10.21,22 Therefore, C5a down-modulates the more potent IL-17A while up-regulating the weaker IL-17F.

Activation of the C5a-C5aR pathway induced intrinsic (mitochondrial) and extrinsic (TNFα, Fas ligand) apoptosis of thymocytes, adrenal medullary and cortical cells.23 C5a inhibited spontaneous human neutrophil apoptosis via PI3-K/Akt signalling pathway.24,25 The local complement-dependent expansion of the effector repertoire results from C5a/C5aR-induced suppression of effector T-cell apoptosis, through PI-3Kγ–dependent alterations in Bcl-2 and Fas expression.26 Finally, upregulated C5a impairs neutrophils phagocytosis, reactive oxygen species (ROS) generation, and chemotactic migration.27 C5a and C5b collectively activate platelets and endothelial cells, with subsequent induction of expression of tissue factor, von Willebrand factor, prothrombinase assembly sites and P-selectin.28 E. coli-induced tissue factor upregulation is mainly dependent on C5a and much less on CD14.29,30 Finally, C5a plays a critical role in sepsis induced cardiomyopathy mainly by down-regulating sarcoplasmic reticulum Ca2+-ATPase and sarcolemma Na+-Ca2+ exchanger, key calcium regulatory proteins in left ventricle cardiomyocytes.31 Endotoxin upregulates C5a-C5aR system in a time-dependent manner subsequently within cerebral endothelium, microglial cells neighbouring the endothelium, and in deeper brain parenchyma.32 C5a triggers microglial cells release of proinflammatory cytokines and phagocytosis capacity, and activates astrocytes.

Role of Anaphylatoxins in the Physiopathology of Coronavirus Diseases

Acute respiratory distress syndrome (ARDS) is a severe complication of coronavirus infection.33–35 There are numerous cytokine-related molecules and pathways relevant to understanding the biological mechanisms underlying acute lung injury after viral infection. Complement activation and C5a play a key role in the development of virus-related ARDS.36 In C57BL/6J mice, mouse-adapted SARS-CoV infection resulted in high-titre virus replication within the lung, induction of inflammatory cytokines and chemokines, and immune cell infiltration within the lung.37 C3 activation products were detected in SARS-CoV-infected mice, but not in control mice, as early as 24 hours post-infection. Complement component 3 deposition was observed in the lungs two to four days following infection in wildtype mice. C3 knocked out mice were protected from SARSCoV-induced weight loss, had reduced lung damage, lung and systemic inflammation, and improved respiratory function. The unaltered kinetics of viral replication in the C3-deficient mice suggested that the protective effects related to the prevention of complement-mediated inflammatory processes and not to reduction of viral titre. In contrast, transgenic mice lacking alternative pathway proteins, Factor B or C4, were not protected from SARS CoV-mediated weight loss, suggesting that inhibition of the complement alternative pathway alone may not be sufficient. This implies that inhibition of C3 or C5 may be required. Likewise, Middle East respiratory syndrome (MERS)-CoV infection in mice caused severe acute respiratory failure and high mortality accompanied by elevated secretion of cytokines and chemokines.38 In this model, complement overactivation was characterized by increased concentrations in sera and lung tissue of C5a and C5b-9. The avian influenza virus H5N1 induced histopathological changes in the lungs are like those observed in SARS.39 In a mouse model of H5N1, complement activates immune effector cells and drives lung inflammation. C3a and C5a increased vascular permeability, activated leukocytes and endothelial cells, up-regulated adhesion molecule and cytokine expression, and induced goblet cell secretion of mucus. In these mice, deposition of C3, C5b-9, and mannose-binding lectin (MBL)-C was observed in lung tissue. Up-regulation of MBL-associated serine protease-2 (MASP-2) and complement receptors C3aR and C5aR was also detected. Specific inhibition of either C3aR or C5a in the infected mice reduced lung damage, inflammation and neutrophil infiltration, and improved survival.40 Upregulation of MASP-2 by extracellular release of the viral N-protein may be a common pathway of complement overaction by coronaviruses.41

Clinical evidence suggests that complement is activated during SARS infection and that the progression of severe pneumonia, acute lung injury, or ARDS in these patients is strongly associated with complement activation.42 Likewise, C5a is associated with the inflammatory response and severe lung damage that occurs in patients infected with the 2009 H1N1 influenza virus.43 It has also been shown that SARS-CoV can directly activate complement via the lectin pathway.44 Cytokine storm is thought to be a key step in the pathogenesis of ARDS following SARS-CoV-2 infection.45 Observational studies have shown evidence for over-activation of the complement pathway in patients with COVID-19. Lung and skin biopsies in patients with severe COVID-19 revealed microvascular damage and microthrombosis consistent with activation of the alternative and lectin pathways.46 Indeed, vascular injuries were associated with extensive of C5b-9, C4d and MASP-2. Clinical studies consistently reported increased levels of sC5b-9 correlating with severity of illness.47–50 Likewise, levels of C5a were gradually increased in serum and bronchoalveolar lavage from asymptomatic patients, patients with pneumonia and those with ARDS.51 Upregulation of the C5a-C5aR axis was sustained for at least 10 days after admission probably as a result of prolonged activation of the lectin and classical pathways. Overactivation of C5a-C5aR axis in the lung contributes directly and indirectly to recruitment of myeloid cells and subsequently to vasculitis and endothelialitis.

Collectively, preclinical and clinical observations suggest that COVID-19, alike other highly pathogenic viral diseases, is a complement-mediated disease, and that modulating the complement cascade may be an effective treatment option.

Complement System Inhibition

Theoretically, options for blocking the complement system may include targeting components of the lectin, classical or alternative pathways, or acting downstream at the level of C3-C3a or C5-C5a.52,53

Preclinical Studies

Main preclinical studies are summarized in Table 2. In E. coli challenged primates, selective inhibition of the C3 convertase prevented TLR-4 mediated inflammatory signals, tissues inflammation, preserved organs function and improved survival.54 These effects were preceded by inhibition of complement activation and deposition in tissues of MDL, C3b and C4b-9. In the same model, inhibition of C3 convertase protected against E. coli sepsis induced lung fibrosis by downregulating profibrogenic and matrix proteins genes.55 In rats with CLP induced sepsis, anti-C5a antibodies significantly reduced systemic and tissues inflammation, restored the coagulation/fibrinolytic balance, and improved survival.56,57 Likewise, in piglets with polymicrobial sepsis, the combination of the C5 inhibitor coversin and of an anti-CD14 significantly attenuated the inflammatory response and disseminated intravascular coagulopathy, improved hemodynamic, organ function and survival.58,59 In mice challenged with lethal load of H5N1, C3aR or C5aR inhibitors prevented neutrophils infiltration in the lung, downregulated local and systemic release of proinflammatory mediators with subsequent attenuation of lung injury and increased survival rate.40 The pharmacological approach of inhibiting C3a-C3aR or C5a-C5aR axes resulted in similar anti-inflammatory effects, organs protection and survival in H7N9 infected non-human primates60 and in MERS-CoV infected mice.38 Monoclonal antibodies that selectively blocked C5a-R1 prevented lung inflammation and injuries in a mice model of inhaled C5a induced acute lung injury mimicking COVID-19.51

Table 2

Preclinical Studies on Complement Inhibition

Authors	Models	Animals	Interventions	Effects
Silasi-Mansat54	Infusion with 1×109 live E coli (LD50 dose)	Baboons	C3 convertase inhibitor: Compstatin was administered as a 10-mg/kg intravenous bolus followed by 60 μg/kg/min continuous infusion	Reduction in inflammatory and hemostatic processes, Improvement in systemic blood pressure and organ function
Silasi-Mansat55	Infusion with 1×109 live E coli (LD50 dose)	Baboons	C3 convertase inhibitor: Compstatin was administered as a 10-mg/kg intravenous bolus followed by 60 μg/kg/min continuous infusion	Reduction in early profibrogenic responses in the lung, including fibroblast differentiation, cell migration and proliferation, and the enhanced production of collagens and other matrix proteins.
Czermak56	Cecal ligation and puncture	Rats	IgG antibody against C5a	Improvement in survival rates Reduction in levels of bacteremia
Laudes57	Cecal ligation and puncture	Rats	Rabbit Anti-Rat C5a	Improvement in survival ratesImprovement in platelet counts, fibrinogen, FVII:C, AT, plasminogen, t-PA, and PAI as well as TAT complexes and D-dimer
Barratt-Due58	Live E. coli sepsis	Pigs	C5 and leukotriene B4 inhibitor Ornithodoros moubata complement inhibitor (OmCI; coversin) alone and combined with anti-CD14	Reduction in proinflammatory mediators and in thrombogenicity,Increase in IL-10, Delayed hemodynamic changes
Skjeflo59	Live E. coli sepsis	Pigs	C5 and leukotriene B4 inhibitor Ornithodoros moubata complement inhibitor (OmCI; coversin) alone and combined with anti-CD14	Improvement in survivalImprovement in pulmonary and systemic hemodynamicReduction in proinflammatory mediators
Sun60	Intratracheal inoculation with 10650% tissue culture infective dose (TCID50) of A/Anhui/1/2013 (H7N9) virus	Green monkeys	Neutralizing specific antihuman C5a antibody (IFX-1)	Reduction in the ALI and systemic inflammationReduction in lung viral replication

Clinical Studies

Main studies on the use of complement factor inhibitors in sepsis or COVID-19 are summarized in Table 3. Small sized observational studies suggested favorable outcomes in patients with sepsis treated with C1 esterase inhibitor.61,62 In a randomized trial, infusions of C1 esterase inhibitor reduced 28-day mortality by 33% from 45%.63 This trial has several limitations including a small sample size (61 patients), the lack of a placebo and of blinding of treatment administration and outcomes assessment. In a patient with sepsis and disseminated intravascular coagulopathy induced thrombotic microangiopathy, treatment with eculizumab, humanized monoclonal antibody that inhibits the cleavage of C5 to C5a and C5b, restored the coagulation/fibrinolytic balance and reduced sepsis associated terminal organs complications.64

Table 3

Summary of Clinical Studies of Complement Inhibition

Authors	Disease	Study Design	Interventions	Main Findings
Fronhoffs 200062	Streptococcal toxic shock syndrome	Case seriesN=7	C1-esterase inhibitor, 6000 to 10,000 U, within the first 24 hours	Marked reduction in vascular leaks and rapid weaning of vasopressor6/7 survivors
Igonin 201263	Adults with sepsis	Open-label randomized controlled studyN=61	C1-esterase inhibitor infusions, 12,000 U	Increased C1-esterase inhibitor functional activityIncreased C3 levelsDecreased C-reactive proteinreduced all-cause mortality (12% vs 45% in control, p = 0.008)
Abe 201764	Sepsis induced coagulopathy with thrombotic microangiopathies	Case reportN=1	Eculizumab 900 mg once a week for 4 weeks	Rapid weaning of mechanical ventilation and vasopressor, and normalization of renal function
Urwyler 202065	Severe COVID-19	Case seriesN=5	Human recombinant C1 esterase inhibitor (conestat alfa), 8400 IU followed by 3 additional doses of 4200 IU in 12-h intervals	Rapid improvement in clinical and laboratory markers of inflammation
Rambaldi 202066	COVID-19 ARDS	Case seriesN=6Retrospective controls	Narsoplimab, human immunoglobulin gamma 4 (IgG4) monoclonal antibody against MASP-2	Rapid decrease in markers of endothelial injury (circulating endothelial cells) and of inflammation (IL-6, IL-8, CRP)
Mastaglio 202067	COVID-19 ARDS	Case reportN=1	AMY-101 intravenously 6-hour loading infusion of 5 mg/kg mg/Kg/day, followed by 13 maintenance doses as 24-h continuous infusions, for a 14-day treatment period	Rapid clinical improvement and reduction in inflammatory biomarkers
Peffault de La Tour48	Severe COVID-19	Case seriesN=8	Eculizumab 3 infusions of 900 to 1200 mg every 4 days	6/8 survivorsSurvivors had rapid clinical improvement and reduction of serum levels of inflammatory biomarkers
Zelek 202068	COVID-19 ARDSWith high circulating levels of terminal complement factors	Case seriesN=5	LFG316 single 1500-mg dose by intravenous infusion	4/5 patients had sustained improvement in clinical state persisting beyond C5 blockade
Laurence 2020	Severe COVID-19With evidence for skin deposition of terminal complement factors	Case seriesN=3		Marked decline in D-dimers and neutrophil counts1 patient had full recovery 1 patient died, and the last one had partial recovery
Diurno 2020	Severe COVID-19	Case seriesN=4	Eculizumab 900 mg Up to 4 weekly infusions	Marked clinical improvement within the first 48 hours after the first administration of eculizumab
Annane 202049	COVID-19 ARDS	Quasi randomizedN=80	Eculizumab single infusions of 900 to 1200 mg were administered intravenously over 45 min on days 1 (within 7 days of confirmed pneumonia or ARDS), 8, 15, and 22	Eculizumab improved day 15 survival 82.9% (95% CI: 70.4%‒95.3%) with eculizumab and 62.2% (48.1%‒76.4%) without eculizumab (Log rank test, P = 0.04)More rapid resolution of inflammation, coagulopathy and organs dysfunctionIncreased secondary bacterial infections
Vlaar 202072	Severe COVID-19	Open-label, randomized phase 2 trial	Vilobelimab, IFX-1, anti-C5a antibody (up to seven doses of 800 mg intravenously) plus best supportive care or best supportive care only	Mortality by 28 days were 13% (95% CI 0–31) for the IFX-1 group and 27% (4–49) for the control group (adjusted hazard ratio for death 0·65 [95% CI 0·10–4·14])There was no difference in PaO2/FiO2 ratio overtime

Cases series have suggested promising effects on clinical outcomes in patients with severe COVID-19 treated with C1 esterase inhibitors,65 MASP-2 monoclonal antibodies,66 compstatin-based complement C3 inhibitor,67 anti-C5 drugs,48,68–70 or C5a-C5aR1 antagonists.41,51 A non-randomized study compared the complement C3 inhibitor AMY-101 and eculizumab -an anti-C5 monoclonal antibody- administered to 3 and 10 patients with severe COVID-19, respectively.71 Both drugs were associated with substantial reduction in circulating biomarkers of inflammation. The C3 inhibitor was associated with faster decrease in neutrophils count and NETosis and increase in lymphocytes and platelets count than eculizumab. Lung function and arterial haematosis improved similarly with both drugs. Unsurprisingly, AMY-101 and not eculizumab resulted in a rapid and sustained decrease in C3a serum levels. Of note, in contrast with AMY-101 effects, eculizumab was associated with transient decrease followed by a rebound in sC5b69 levels and residual AP-mediated complement haemolytic activity, suggesting transient and incomplete terminal pathway inhibition. These findings would support that in COVID-19, targeting C3 or its upstream activators may provide more benefits than the sole inhibition of C5-C5a-C5a-R axis via the regulation beyond the cytokine storm, of the Net generation and thrombotic microangiopathy. A quasi-randomized trial has investigated intravenous administration of 900mg of eculizumab at day 1, 8, 15 and 22 of ICU admission, in 80 critically ill COVID-19.49 Preliminary data found that these doses resulted in incomplete and transient inhibition of terminal pathway, and subsequent patients received higher doses at more frequent infusions. Eculizumab treated patients were more likely to survive at day-15 and day 28 and had prolonged survival time as compared to control. Eculizumab was associated with faster improvement in arterial oxygen tension and tissue oxygenation, more rapid increase in platelets count and prothrombin time, better renal function, and steeper decline in circulating levels of IL-6, IL-17 and IFN-α2. There were more patients with secondary bacterial infections in the eculizumab group versus controls. While levels of CH50 and sC5b-9 were dramatically decreased by eculizumab, levels of C5a did not differ from those of eculizumab-free patients.50 The incomplete terminal pathway inhibition by eculizumab in patients with severe COVID-19 is unclear. The strong activation of complement may have played a role in insufficient inhibition of the terminal complement pathway by eculizumab. Targeting C5a or C5a-R1 may offer the advantage over C5 antagonists, to preserve the formation of the membrane complex attack which is crucial to kill bacteria and to provide complete blockade of the anaphylatoxin C5a. The favorable benefit to risk ratio suggested in observational studies and small sized controlled studies should be tempered off by serious limitations inherent to the design of these studies, including intermediate outcomes and not patient-centered outcome and limited controls for selection and confounding biases. A proof-of-concept, phase 2 study has investigated IFX-1 - a selective antagonist of C5a- in 30 adults with severe COVID-19.72 There was no significant difference in changes in the ratio of arterial tension to the fraction of inspired oxygen (PaO2/FiO2) between IFX-1 treated and free patients (17% vs 41%; difference –24% [95% CI –58 to 9], p=0.15). Likewise, there were no difference in mortality by 28 days, 13% (95% CI 0–31) for IFX-1 and 27% (4–49) for controls (HR for death 0.65 [95% CI 0.10–4.14]). Patients in the experimental arm were more likely to have normalized lymphocytes count by day 15 (13 /15 [87%] versus 7/15 [47%], p=0.05). This adaptive design trial has now progressed to a Phase 3, placebo-controlled stage. Several ongoing randomized trials are investigating in severe COVID-19, the anti-C5 drugs zilucoplan,73,74 eculizumab,75 ravulizumab,76–78 the C3 inhibitors AMY-10179 and APL-9,80 the C1 esterase inhibitor conestat alpha,81–83 the novel peptide RLS-0071,84 the MASP-2 inhibitor narsoplimab.85 These trials are of variable size (ranging from 32 to 1500 participants) and most often compare active treatment to best supportive care.

Conclusion

Alike bacterial sepsis, severe COVID-19 is characterized by overactivation of the terminal complement pathway that contributes to airways and lung inflammation and injuries, disruption of endothelial function with subsequent spreading of the excessive inflammatory response to the periphery and imbalanced coagulation/fibrinolysis resulting in organs failure and eventually death. Collectively experimental data and preliminary results from observational studies shed light on the potential benefits from complement inhibition in severe COVID-19. Ongoing randomized trials should confirm a favorable benefit to risk ratio for complement inhibitors, and should precise the optimal targets in the complement cascade. Awaiting final results from these trials, complement inhibition should not be part of the routine management of patients with COVID-19.