Benjamin Tuck1, Elizabeth Watkin2, Maria Forsyth3, Anthony Somers3, Mahdi Ghorbani3, Laura L Machuca4. 1. Curtin Corrosion Centre, WA School of Mines: Minerals, Energy and Chemical Engineering, Curtin University, Kent Street, Bentley, WA, 6102, Australia. 2. Curtin Medical School, Curtin University, Kent Street, Bentley, WA, 6102, Australia. 3. Institute for Frontier Materials, Deakin University, Burwood, VIC, 3217, Australia. 4. Curtin Corrosion Centre, WA School of Mines: Minerals, Energy and Chemical Engineering, Curtin University, Kent Street, Bentley, WA, 6102, Australia. L.Machuca2@curtin.edu.au.
Abstract
Chemical biocides remain the most effective mitigation strategy against microbiologically influenced corrosion (MIC), one of the costliest and most pervasive forms of corrosion in industry. However, toxicity and environmental concerns associated with these compounds are encouraging the development of more environmentally friendly MIC inhibitors. In this study, we evaluated the antimicrobial effect of a novel, multi-functional organic corrosion inhibitor (OCI) compound, cetrimonium trans-4-hydroxy-cinnamate (CTA-4OHcinn). Attachment of three bacterial strains, Shewanella chilikensis, Pseudomonas balearica and Klebsiella pneumoniae was evaluated on wet-ground (120 grit finish) and pre-oxidised carbon steel surfaces (AISI 1030), in the presence and absence of the new OCI compound. Our study revealed that all strains preferentially attached to pre-oxidised surfaces as indicated by confocal laser scanning microscopy, scanning electron microscopy and standard colony forming unit (CFU) quantification assays. The inhibitor compound at 10 mM demonstrated 100% reduction in S. chilikensis attachment independent of initial surface condition, while the other two strains were reduced by at least 99.7% of the original viable cell number. Our results demonstrate that CTA-4OHcinn is biocidal active and has promise as a multifunctional, environmentally sound MIC inhibitor for industrial applications.
Chemical biocidn class="Chemical">es remain the most effective mitigation strategy against microbiologically influenced corrosion (MIC), one of the costliest and most pervasive forms of corrosion in industry. However, toxicity and environmental concerns associated with these compounds are encouraging the development of more environmentally friendly MIC inhibitors. In this study, we evaluated the antimicrobial effect of a novel, multi-functional organic corrosion inhibitor (OCI) compound, cetrimonium trans-4-hydroxy-cinnamate (CTA-4OHcinn). Attachment of three bacterial strains, Shewanella chilikensis, Pseudomonas balearica and Klebsiella pneumoniae was evaluated on wet-ground (120 grit finish) and pre-oxidised carbon steel surfaces (AISI 1030), in the presence and absence of the new OCI compound. Our study revealed that all strains preferentially attached to pre-oxidised surfaces as indicated by confocal laser scanning microscopy, scanning electron microscopy and standard colony forming unit (CFU) quantification assays. The inhibitor compound at 10 mM demonstrated 100% reduction in S. chilikensis attachment independent of initial surface condition, while the other two strains were reduced by at least 99.7% of the original viable cell number. Our results demonstrate that CTA-4OHcinn is biocidal active and has promise as a multifunctional, environmentally sound MIC inhibitor for industrial applications.
Authors: Dirk Hauck; Ines Joachim; Benjamin Frommeyer; Annabelle Varrot; Bodo Philipp; Heiko M Möller; Anne Imberty; Thomas E Exner; Alexander Titz Journal: ACS Chem Biol Date: 2013-06-28 Impact factor: 5.100
Authors: Mahdi Ghorbani; Jhonatan Soto Puelles; Maria Forsyth; Rainier A Catubig; Leigh Ackland; Laura Machuca; Herman Terryn; Anthony E Somers Journal: J Phys Chem Lett Date: 2020-11-10 Impact factor: 6.475
Authors: Benjamin Tuck; Nadia Leinecker; Elizabeth Watkin; Anthony Somers; Maria Forsyth; Laura L Machuca Journal: Front Bioeng Biotechnol Date: 2022-01-21
Authors: Benjamin Tuck; Silvia J Salgar-Chaparro; Elizabeth Watkin; Anthony Somers; Maria Forsyth; Laura L Machuca Journal: Microorganisms Date: 2022-06-24