BACKGROUND: Prurigo nodularis (PN) is a highly pruritic disease that significantly impairs patient quality of life. Although the mechanism that causes pruritus is not clear, one hypothesis argues that neural hyperplasia, mast cell, and Merkel cell neurite complexes may be associated with PN pathogenesis. OBJECTIVE: The objective of this study was to analyze whether special staining outcomes differed depending on the presence of atopic dermatitis (AD) and treatment response. METHODS: A total of 209 patients diagnosed with PN was analyzed retrospectively. Patients were divided into two groups according to presence or past history of AD and by treatment response. Histopathologic features were obtained using the following stains: Giemsa, S-100, neuron-specific enolase, cytokeratin (CK)-20, CAM5.2, and CK8/CK18. RESULTS: A total of 126 patients (60.29%) had AD, and 68 (32.54%) showed clinical improvement. There were no statistically significant differences in the staining results between the PN groups with AD (PN c AD) and without AD (PN s AD). Additionally, there were no statistically significant differences in staining results between the improved and non-improved groups. CONCLUSION: Implementing the special stains helped to identify PN pathogenesis. Because there were no statistically significant differences in the special stain results between the improved and non-improved groups, we conclude that mast cell proliferation, neural hyperplasia, and Merkel cell hyperplasia may not have a significant effect on treatment response.
BACKGROUND: Prurigo nodularis (PN) is a highly pruritic disease that significantly impairs patient quality of life. Although the mechanism that causes pruritus is not clear, one hypothesis argues that neural hyperplasia, mast cell, and Merkel cell neurite complexes may be associated with PN pathogenesis. OBJECTIVE: The objective of this study was to analyze whether special staining outcomes differed depending on the presence of atopic dermatitis (AD) and treatment response. METHODS: A total of 209 patients diagnosed with PN was analyzed retrospectively. Patients were divided into two groups according to presence or past history of AD and by treatment response. Histopathologic features were obtained using the following stains: Giemsa, S-100, neuron-specific enolase, cytokeratin (CK)-20, CAM5.2, and CK8/CK18. RESULTS: A total of 126 patients (60.29%) had AD, and 68 (32.54%) showed clinical improvement. There were no statistically significant differences in the staining results between the PN groups with AD (PN c AD) and without AD (PN s AD). Additionally, there were no statistically significant differences in staining results between the improved and non-improved groups. CONCLUSION: Implementing the special stains helped to identify PN pathogenesis. Because there were no statistically significant differences in the special stain results between the improved and non-improved groups, we conclude that mast cell proliferation, neural hyperplasia, and Merkel cell hyperplasia may not have a significant effect on treatment response.
Authors: Elke Weisshaar; Jacek C Szepietowski; Ulf Darsow; Laurent Misery; Joanna Wallengren; Thomas Mettang; Uwe Gieler; Torello Lotti; Julien Lambert; P Maisel; Markus Streit; Malcolm W Greaves; Andrew J Carmichael; Erwin Tschachler; Johannes Ring; Sonja Ständer Journal: Acta Derm Venereol Date: 2012-09 Impact factor: 4.437
Authors: Laura Saporito; Ada Maria Florena; Claudia Colomba; Diego Pampinella; Paola Di Carlo Journal: J Med Microbiol Date: 2009-08-06 Impact factor: 2.472
Authors: E Garbuzenko; A Nagler; D Pickholtz; P Gillery; R Reich; F-X Maquart; F Levi-Schaffer Journal: Clin Exp Allergy Date: 2002-02 Impact factor: 5.018
Authors: David A Groneberg; Frauke Serowka; Nora Peckenschneider; Metin Artuc; Andreas Grützkau; Axel Fischer; Beate M Henz; Pia Welker Journal: J Neuroimmunol Date: 2005-04 Impact factor: 3.478