Kristina Navrazhina1, Sandra Garcet2, John W Frew2, Xiuzhong Zheng2, Israel Coats2, Emma Guttman-Yassky3, James G Krueger4. 1. Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York; Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, New York. 2. Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York. 3. Laboratory of Inflammatory Skin Diseases, Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York. 4. Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York. Electronic address: jgk@rockefeller.edu.
Abstract
BACKGROUND: Although hidradenitis suppurativa (HS) shares some transcriptomic and cellular infiltrate features with psoriasis, their skin proteome remains unknown. OBJECTIVE: To define and compare inflammatory protein biomarkers of HS and psoriasis skin. METHODS: We assessed 92 inflammatory biomarkers in HS (n = 13), psoriasis (n = 11), and control skin (n = 11) using Olink high-throughput proteomics. We also correlated HS skin and blood biomarkers using proteomics and RNA sequencing. RESULTS: We identified 57 differentially expressed proteins (DEPs) in lesional psoriasis and 64 DEPs in lesional HS skin, compared to healthy controls. Both HS and psoriasis lesional skin demonstrated a significant upregulation of T helper 1 and T helper 17 proteins. Healthy-appearing perilesional HS skin had 63 DEPs compared to healthy controls. Nonlesional HS and psoriasis skin had 24 and 7 DEPs, respectively, compared to healthy controls. Tumor necrosis factor and 8 other proteins were significantly correlated with clinical severity in perilesional HS skin (2 cm from a nodule). LIMITATIONS: Inclusion of only moderate-to-severe patients and the cohort size. CONCLUSION: HS has a greater inflammatory profile and is more diffusely distributed compared with psoriasis. Proteins correlated with disease severity are potential disease mediators. Perilesional skin is comparably inflamed to lesional skin, suggesting the need to treat beyond skin nodules.
BACKGROUND: Although hidradenitis suppurativa (HS) shares some transcriptomic and cellular infiltrate features with psoriasis, their skin proteome remains unknown. OBJECTIVE: To define and compare inflammatory protein biomarkers of HS and psoriasis skin. METHODS: We assessed 92 inflammatory biomarkers in HS (n = 13), psoriasis (n = 11), and control skin (n = 11) using Olink high-throughput proteomics. We also correlated HS skin and blood biomarkers using proteomics and RNA sequencing. RESULTS: We identified 57 differentially expressed proteins (DEPs) in lesional psoriasis and 64 DEPs in lesional HS skin, compared to healthy controls. Both HS and psoriasis lesional skin demonstrated a significant upregulation of T helper 1 and T helper 17 proteins. Healthy-appearing perilesional HS skin had 63 DEPs compared to healthy controls. Nonlesional HS and psoriasis skin had 24 and 7 DEPs, respectively, compared to healthy controls. Tumor necrosis factor and 8 other proteins were significantly correlated with clinical severity in perilesional HS skin (2 cm from a nodule). LIMITATIONS: Inclusion of only moderate-to-severe patients and the cohort size. CONCLUSION: HS has a greater inflammatory profile and is more diffusely distributed compared with psoriasis. Proteins correlated with disease severity are potential disease mediators. Perilesional skin is comparably inflamed to lesional skin, suggesting the need to treat beyond skin nodules.
Authors: Christopher M Elitt; Sabrina L McIlwrath; Jeffery J Lawson; Sacha A Malin; Derek C Molliver; Pamela K Cornuet; H Richard Koerber; Brian M Davis; Kathryn M Albers Journal: J Neurosci Date: 2006-08-16 Impact factor: 6.167
Authors: T Nakamura; T Nishizawa; M Hagiya; T Seki; M Shimonishi; A Sugimura; K Tashiro; S Shimizu Journal: Nature Date: 1989-11-23 Impact factor: 49.962
Authors: S Ribero; A Ramondetta; G Fabbrocini; V Bettoli; C Potenza; A Chiricozzi; M Licciardello; A V Marzano; L Bianchi; G Rozzo; L Fania; C Marasca; G Odorici; A Mambrin; C Moltrasio; R D Caposiena Caro; N Skroza; P Quaglino; N Siliquini; P Dapavo Journal: J Eur Acad Dermatol Venereol Date: 2021-03-09 Impact factor: 6.166
Authors: J W Frew; K Navrazhina; A S Byrd; A Garg; J R Ingram; J S Kirby; M A Lowes; H Naik; V Piguet; E P Prens Journal: Br J Dermatol Date: 2019-08-29 Impact factor: 11.113
Authors: David Quarcoo; Tanja C Fischer; Nora Peckenschneider; David A Groneberg; Pia Welker Journal: J Occup Med Toxicol Date: 2009-04-22 Impact factor: 2.646
Authors: Vladimir V Sobolev; Anna G Soboleva; Elena V Denisova; Eva A Pechatnikova; Eugenia Dvoryankova; Irina M Korsunskaya; Alexandre Mezentsev Journal: Biomedicines Date: 2022-03-07