Root colonization and rhizospheric community structure of Arbuscular Mycorrhizal Fungi in BADH transgenic maize BZ-136 and its recipient under salt stress and neutral soil.

Betaine aldehyde dehydrogenase (BADH) transgenic maize has a capability to grow under drought and salt stress; the risk of planting BADH transgenic maize on symbiotic microorganisms remains problematic, however. A pot experiment was carried out to assess the impact of BADH transgenic maize BZ-136 on arbuscular mycorrhizal fungi (AMF) colonization in root and community structure in rhizosphere soil compared with that of parental maize Zheng58 in neutral and saline-alkaline soil. Microscope observation found that BZ-136 only had a significantly reduced effect on AMF colonization at the elongation stage (9-14%). High-throughput sequencing analysis revealed that the AMF taxonomic composition kept consistency at the genus level between transgenic BZ-136 and non-transgenic parental Zheng58. NMDS analysis verified the slight difference in community structure between BZ-136 and Zheng58 presented an agrotype-dependent pattern. AMF community indices showed that BZ-136 had a higher richness at the flowering stage in saline-alkaline soil and had a higher diversity at the mature stage in neutral soil. Heatmap analysis also illuminated AMF community structure of transgenic maize at species level was similar to that of non-transgenic maize. In summary, cropping transgenic BADH maize has minor or transient effects on AMF colonization and rhizospheric soil AMF community structure, while agrotype has a stronger effect on AMF community structure.