Xiyang Wu1,2, Yue Chen1,2, Chenggang Li1, Xin Zhang1, Xinqiu Tan1,2, Liang Lv3, Yong Liu1,2, Deyong Zhang1,2. 1. Hunan Academy of Agricultural Sciences, State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Changsha, 410125, China. 2. Long Ping Branch, Graduate School of Hunan University, Changsha, 410125, China. 3. Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture, P.R. China, and Hubei Province Key Laboratory for Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection & Soil Science, Hubei, Academy of Agricultural Sciences, Wuhan, 430068, China.
Abstract
BACKGROUND: GroEL, which is a chaperone, plays a key role in maintaining protein homeostasis and, among other functions, serves to prevent protein misfolding and aggregation. In addition, the GroEL protein also has a significant effect on enhancing plant resistance and inhibiting plant diseases. However, the function of the GroEL protein in the inhibition of rice blast remains unknown. RESULT: Field experiment results show that photosynthetic bacteria PSB-06 have a good control effect on M. oryzae. PSB-06 also can promote rice growth and enhance the stress resistance. A GroEL protein which was separated and purified from photosynthetic bacteria had a significant antagonistic effect on appressorial formation and pathogenicity of M. oryzae, meanwhile transcriptional analysis demonstrated that the GroEL protein could improve the expression of defense gene of rice. CONCLUSION: Our results show that the photosynthetic bacteria Rhodopseudomonas palustris significantly controls rice blast disease. Its action involves an extracellular GroEL protein, which inhibits appressoria formation, antagonizes the pathogenicity of Magnaporthe oryzae and promotes a host defense response. The research results provide evidence of the potential of this photosynthetic bacterium as biocontrol agent at least for rice blast control. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
BACKGROUND: GroEL, which is a chaperone, plays a key role in maintaining protein homeostasis and, among other functions, serves to prevent protein misfolding and aggregation. In addition, the GroEL protein also has a significant effect on enhancing plant resistance and inhibiting plant diseases. However, the function of the GroEL protein in the inhibition of rice blast remains unknown. RESULT: Field experiment results show that photosynthetic bacteria PSB-06 have a good control effect on M. oryzae. PSB-06 also can promote rice growth and enhance the stress resistance. A GroEL protein which was separated and purified from photosynthetic bacteria had a significant antagonistic effect on appressorial formation and pathogenicity of M. oryzae, meanwhile transcriptional analysis demonstrated that the GroEL protein could improve the expression of defense gene of rice. CONCLUSION: Our results show that the photosynthetic bacteria Rhodopseudomonas palustris significantly controls rice blast disease. Its action involves an extracellular GroEL protein, which inhibits appressoria formation, antagonizes the pathogenicity of Magnaporthe oryzae and promotes a host defense response. The research results provide evidence of the potential of this photosynthetic bacterium as biocontrol agent at least for rice blast control. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.