Xijun Du1,2, Weigang Xu3,4, Chaojun Peng2, Chunxin Li2, Yu Zhang2, Lin Hu2. 1. College of Agronomy, Northwest A&F University, Yangling, Shanxi, 712100, Xianyang, China. 2. Institute of Crop Molecular Breeding/National Engineering Laboratory of Wheat/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area/Ministry of Agriculture/Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China. 3. College of Agronomy, Northwest A&F University, Yangling, Shanxi, 712100, Xianyang, China. xuwg1958@163.com. 4. Institute of Crop Molecular Breeding/National Engineering Laboratory of Wheat/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area/Ministry of Agriculture/Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China. xuwg1958@163.com.
Abstract
BACKGROUND: Powdery mildew (PM), one of the major diseases in wheat, severely damages yield and quality, and the most economical and effective way to address this issue is to breed disease-resistant cultivars. Accordingly, 371 landraces and 266 released cultivars in Henan Province were genotyped by a 660 K microarray and phenotyped for adult plant resistance (APR) to PM from 2017 to 2020, and these datasets were used to conduct multilocus genome-wide association studies (GWASs). RESULTS: Thirty-six varieties showed stable APR in all the environments, and eleven quantitative trait nucleotides (QTNs) were found by multiple methods across multiple environments and best linear unbiased prediction (BLUP) values to be significantly associated with APR. Among these stable QTNs, four were previously reported, three were newly discovered in this study, and the others need to be further investigated. The major and newly discovered QTN, Qpm-3BL, was located at chr03BL_AX-109,052,670, while another newly discovered QTN, Qpm-1BL, was located between chr01BL_AX-108,771,002 and chr01BL_AX-110,117,322. Five and eight landraces were identified to be resistant based on Qpm-1BL (haplotype TC) and Qpm-3BL (allele T), respectively. To validate Qpm-3BL, a new kompetitive allele-specific PCR (KASP) marker was developed to scan 155 F2 individuals, and the average resistance score supported the value of Qpm-3BL in marker-assisted breeding. Near Qpm-3BL, PmBMYD was identified by KEGG, gene expression and comparative genomics analyses to be a candidate. Its resistance mechanism may involve gene tandem repeats. CONCLUSIONS: This study reveals a previously unknown gene for PM resistance that is available for marker-assisted breeding.
BACKGROUND:Powdery mildew (PM), one of the major diseases in wheat, severely damages yield and quality, and the most economical and effective way to address this issue is to breed disease-resistant cultivars. Accordingly, 371 landraces and 266 released cultivars in Henan Province were genotyped by a 660 K microarray and phenotyped for adult plant resistance (APR) to PM from 2017 to 2020, and these datasets were used to conduct multilocus genome-wide association studies (GWASs). RESULTS: Thirty-six varieties showed stable APR in all the environments, and eleven quantitative trait nucleotides (QTNs) were found by multiple methods across multiple environments and best linear unbiased prediction (BLUP) values to be significantly associated with APR. Among these stable QTNs, four were previously reported, three were newly discovered in this study, and the others need to be further investigated. The major and newly discovered QTN, Qpm-3BL, was located at chr03BL_AX-109,052,670, while another newly discovered QTN, Qpm-1BL, was located between chr01BL_AX-108,771,002 and chr01BL_AX-110,117,322. Five and eight landraces were identified to be resistant based on Qpm-1BL (haplotype TC) and Qpm-3BL (allele T), respectively. To validate Qpm-3BL, a new kompetitive allele-specific PCR (KASP) marker was developed to scan 155 F2 individuals, and the average resistance score supported the value of Qpm-3BL in marker-assisted breeding. Near Qpm-3BL, PmBMYD was identified by KEGG, gene expression and comparative genomics analyses to be a candidate. Its resistance mechanism may involve gene tandem repeats. CONCLUSIONS: This study reveals a previously unknown gene for PM resistance that is available for marker-assisted breeding.
Authors: Simon G Krattinger; Evans S Lagudah; Wolfgang Spielmeyer; Ravi P Singh; Julio Huerta-Espino; Helen McFadden; Eligio Bossolini; Liselotte L Selter; Beat Keller Journal: Science Date: 2009-02-19 Impact factor: 47.728
Authors: John W Moore; Sybil Herrera-Foessel; Caixia Lan; Wendelin Schnippenkoetter; Michael Ayliffe; Julio Huerta-Espino; Morten Lillemo; Libby Viccars; Ricky Milne; Sambasivam Periyannan; Xiuying Kong; Wolfgang Spielmeyer; Mark Talbot; Harbans Bariana; John W Patrick; Peter Dodds; Ravi Singh; Evans Lagudah Journal: Nat Genet Date: 2015-11-09 Impact factor: 38.330